利用基质辅助激光解吸附飞行时间质谱(MALDI–TOFMS)和反相高效液相色谱(RP-HPLC)分析技术探索和完善快速、准确的分析植物多酚结构与含量的方法,筛选杨梅、茶叶和柚子中能用于药物中间体和保健品开发的植物多酚种类,得到如下研究结果: 1.利用RP-HPLC快速、准确的测定柚子果皮中4种具有良好生理学和药理学活性的多酚化合物含量。结果表明,柚子果皮总酚含量为23.45~26.97mg/g(儿茶素当量),4种化合物含量分别为4.02~4.84mg/g(柚皮苷),2.41~2.90mg/g(对羟基肉桂酸),0.16~0.31mg/g(橙皮素),0.010~0.012mg/g(桔皮素);当体...Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI–TOF MS) and reversed-phase high performance liquid chromatography (HPLC) are applied to establish the methods for characterization of plant polyphenolics and to separate phenolic compounds with excellent physiological and pharmacological activities in Citrus grandis, Myrica rubra and Camellia sinensis. The results ...学位:理学硕士院系专业:生命科学学院生物学系_生态学学号:2172006115227