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Studies on the proteome of Echinococcus multilocularis protoscoleces

Abstract

本文运用蛋白质组学中的双向电泳分离技术、质谱分析技术和蛋白质信息学方法及免疫蛋白质组学中的Western-blotting技术主要对欧洲多房棘球绦虫原头节蛋白及抗原表达谱进行研究。利用ImageMaster软件分析欧洲多房棘球绦虫原头节的双向电泳图谱,共分离到485±18个蛋白斑点,这些蛋白质斑点大部分分子量介于10-120kD、等电点3.5-9的区域。挑选出其中丰度较高的蛋白斑点进行质谱鉴定,结果鉴定出一些与寄生虫生长、发育、运动、调控相关的蛋白,包括钙网蛋白、微管蛋白、转醇酮酶、硫氧还过氧化物酶、谷胱甘肽S转移酶、HSP90、HSP20、HSP70、肌动蛋白结合与切割蛋白(actin-bi...The proteins of Europe Echinococcus multilocularis protoscolece were analyzed by two-dimension electrophoresis (2-DE) separating technique, Western-blotting, Mass spectrometry (MS) technique, and protein bioinformatics. After analyzed 2-DE pattern by ImageMaster software, about 485±18 protein spots were acquired, their molecular weight were about 10-120kDa and the isoelectric points were about 3.5...学位:理学硕士院系专业:生命科学学院生物学系_动物学学号:20042613

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