根结线虫是重要的植物内寄生线虫。本论文以同安农田采集的番茄病根中爪哇根结线虫为实验材料，对病根进行解剖，收集成虫、二期幼虫和卵块。通过对根结线虫胚胎发育和生活史中各期虫体形态的观察，进一步定种为爪哇根结线虫。通过感染室内土培番茄，使得爪哇根结线虫在实验室得到进一步的扩大培养，为后续实验的进行提供了材料。利用rRNA基因间隔序列的保守性引物对来自不同宿主的爪哇根结线虫和南方根结线虫的rDNA-ITS1和rDNA-ITS2序列进行了扩增，与Genebank中根结线虫的ITS1序列在clustalX软件中进行了分析，构建系统发生树和遗传距离矩阵。分析可知，来源于番茄根内和蒲公英根内的爪哇根结线虫rD...Root-knot nematodes are important endo-parasites in economic plants. The roots of tomatoes infected with M. javanica were got from the field of Tong’an rural area and roots were dissected.The egg masses, pre-parasitic juveniles and female adults of M. javanica were collected in the lab.The embryo development and different stages of life cycle of root-knot nematode were observed. In order to obtain...学位：理学硕士院系专业：生命科学学院生物学系_动物学学号：20032601
