啤酒酵母是保证啤酒质量的关键，因此优良啤酒酵母菌种的选育具有重要的意义。本文在含甲磺隆（SM）的培养基上分离抗SM自发突变酵母菌株；用氩激光、甲基磺酸乙酯和亚硝酸诱变啤酒酵母（Saccharomycescerevisiae）后，在含175mg·L-1双乙酰或0.01%2-脱氧葡萄糖的培养基上分离突变株；筛选具有发酵度较高、总高级醇生成量适中、低产双乙酰、低产乙醛等特性，且遗传性稳定的优良菌株，并测定了优良菌株的转化酶和双乙酰还原酶的活性，以及运用RAPD分子标记技术对优良突变株及其亲株进行RAPD分析。 从实验室保藏的啤酒工业酵母菌种分离SM抗性自发突变株，筛选得到几株优良的突变株。其中SM...Brewer’s yeast is the key factor that guarantes the quality of beer. So the selection of the fine strains is significant. The sulfometuron methyl (SM)-resistant brewer’s yeast spontaneous mutants were isolated on the the plate agar containing SM. Saccharomyces aerevisiae strains were mutated by argon-laser, ethylmethane sulfonate (EMS) and nitrous acid, respectively. The mutants were separated on ...学位：理学硕士院系专业：生命科学学院生物学系_发育生物学学号：2005130205
