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A preliminary study on relation of seven metals content with humen semen quality

Abstract

本实验通过电感耦合等离子体质谱法(ICP-MS)测定精浆中七种金属(铅、镉、铬、锰、铜、砷、硒)的含量并检测精浆酸性磷酸酶(ACP)和α-葡萄糖苷酶的活性,与精液各项指标(精子密度、精子活动率、精子正常形态率等)进行相关性分析。得到以下结果:1.金属元素含量与精子活动率之间存在负相关性。精子活动率越高的样品中,金属含量越低;除镉元素外,其他元素含量与精子密度呈负相关。其中:铜、砷、铅三种元素与密度呈显著相关(PCu=0.002,PMn=0.007,PPb=0.009);除硒、镉外,其他五种元素在精子形态异常的组中其含量高于正常样品组,但没有统计学差异;三种精液指标皆异常与正常之间只有锰、砷两元...In this study, we determined the levels of seven metals (lead, cadmium, chromium, manganese, copper, arsenic, selenium) in human semen by inductively coupled plasma mass spectrometry (ICP-MS) and analyzed the correlations between the mental elements and parameters of semen (sperm density, sperm activity rate, and normal morphological sperm rate). We also detected two enzymes activities [Acid phosp...学位:理学硕士院系专业:生命科学学院生物学系_生物化学与分子生物学学号:K20042602

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