unknown

Gene Cloning and Heterogenous Expression of Solubale Hydrogenase from Chromatium vinosum

Abstract

氢酶广泛存在于原核生物和一些简单的真核生物中,它催化分子氢的形成以及氢的氧化:H2↔2H++2e-。光合细菌ChromatiumvinosumDSM185含有一种可溶性氢酶(SH)。它由五个亚基(HoxH,HoxF,HoxY,HoxU和HoxE)组成:HoxH(β亚基)含有由Ni、Fe原子组成的活性中心,HoxF(α亚基)含有FMN、两个[Fe-S]簇以及可能的NADH还原活性位点,HoxY(δ亚基)和HoxU(γ亚基)都含有[Fe-S]簇,HoxE亚基可能参与了电子传递。可溶性氢酶与蓝细菌中的双向氢酶具有相似的结构和生理功能。依据氢酶的保守序列设计引物,通过PCR扩增,依次克隆了...Hydrogenases, which catalyzes the reversible reation: H2 ↔2H+ + 2e-, have been found in many microorganisms. Thephotosynthetic bacterium Chromatium vinosum DSM185 containeda soluble hydrogenase(SH).The SH consists of five subunits.HoxH(βsubunit) is a NiFe-containing subunit. HoxE is likelyinvolved in electron transfer. HoxF(αsubunit) contains FMN ,two [Fe-S] clusters, and the likely active s...学位:理学硕士院系专业:生命科学学院生物学系_微生物学学号:20022603

    Similar works