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Preparation and Immunoassay of Monoclonal Antibody against Bioactive Glycyrrhizic Acid of Traditional Chinese Medicine

Abstract

现代研究表明,甘草酸具有抗肿瘤、抗炎、抗病毒、增强免疫功能等作用。甘草酸是甘草中最重要的活性成分之一,它常作为甘草及其制剂的定量指标,可用于评价药材、成药的质量、制剂的稳定性、优劣制订药品质量标准等。因此,对甘草酸的定量分析在中药的研究上具有重要的现实意义。 本研究在制备甘草酸人工抗原的基础上,通过杂交瘤技术制备抗甘草酸的单克隆抗体,建立了检测甘草酸的酶联免疫吸附法(ELISA)。本实验所取得的主要结果如下: (1)采用高碘酸氧化法合成的免疫抗原GA-BSA和包被抗原GA-HSA,经基质辅助激光解吸飞行时间质谱(MALDI-TOF-MS)鉴定,在GA-BSA和GA-HSA复合物中,半抗原G...Glycyrrhizic acid (GA) is one of the most important bioactive components in Licorice. Modern research has indicated that GA has extensive pharmaceutical properties such as anti-tumor activity, anti-inflammatory, anti-viral activity, stimulating immune function. It usually serves as a quantification index for Licorice and related pharmaceuticals. Therefore, the quantification of GA has great signif...学位:理学硕士院系专业:生命科学学院生物学系_细胞生物学学号:2012005130206

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