生物钟是广泛存在于蓝藻、植物和动物细胞中的内源计时系统，它对各种代谢和行为的实时调控使机体能更好地适应环境日变化（如光照、温度和湿度等）。生物钟一般具有三个主要特点，即在稳恒条件下生物钟仍然能以近似24h的周期自由运行；光/暗和温度信号能重置生物钟时相；生物钟周期长度具有温度补偿性。蓝藻是具有生物钟的最简单生物，蓝藻生物钟的核心是kai基因簇及其编码的蛋白KaiA、KaiB和KaiC，这三种蛋白相互作用调节KaiC的磷酸化状态，从而产生昼夜节律信息。KaiC的磷酸化循环是昼夜节律的起搏器，调控包括kai基因在内的相关基因的节律性表达。为了研究铜绿微囊藻中Kai蛋白的表达节律，我们将三种kai基...The circadian clock is an endogenous cellular mechanism that allows organisms to temporally regulate gene expression and thereby regulate complex biological processes as a function of time. Temporal organization of cellular functions allows organisms to capitalize on the environmental condition at any given time of day. In any organism, biological phenomena defined as a circadian clock have three ...学位：理学硕士院系专业：生命科学学院生物化学与生物技术系_生物化学与分子生物学学号：20042612
