1、将副溶血弧菌培养至对数生长期，用天然海水洗脱，调整菌浓度至107cells/mL左右后置于28℃恒温培养箱进行饥饿试验。结果表明：在饥饿初期细菌总数、CFU和活菌数都有较大幅度上升；在饥饿中、后期，细菌总数、CFU和活菌数都呈下降趋势，其中总菌数与活菌数缓慢下降，而CFU数降低速度较快。结晶紫染色观察的结果表明：饥饿前副溶血弧菌呈短杆状，染色均匀；饥饿后许多细胞中央出现染色较浅的区域，而且细胞形态也变为椭圆形。以对数生长期的副溶血弧菌为对照，对饥饿60d的副溶血弧菌进行热处理和紫外线处理。结果表明饥饿60d的副溶血弧菌对热和紫外线更敏感。用间接ELISA测定饥饿前后副溶血弧菌的最低检测限，...1、 Pathogenic Vibrio parahaemolyticus in log-phase were collected and the concentration was adjusted to 107cells/ mL in natural sea water for starvation studies. The results showed that: all of the total bacteria number 、viable bacteria number and CFU number of V. parahaemolyticus increased remarkably at the initial starvation stages; all of the total bacteria number 、viable bacteria number and CF...学位：博士后院系专业：化学化工学院化学系_化学学号：BH1700003
