Screening and Mutation of Wild Fungi for Enhanced Cellulase’s Production and Optimization of Culture Condition by RSM

Abstract

纤维素是植物细胞壁的主要成分,广泛存在于自然界中,是世界上最丰富、最廉价的可再生资源,纤维素被彻底分解而无污染的一条有效途径便是利用纤维素酶将它水解为可发酵糖类。纤维素酶的生产主要是利用微生物发酵法,但一直以来,由于菌种选育和生产技术进展不大、酶活力低、成本高等原因,没有形成大规模生产。 本文采用甲基磺酸乙酯(EthylMethylSulfonate,EMS)与UV-irradation结合的方法,对野生菌进行诱变,两轮诱变后,筛选到菌株EU2-77,其产生的胞外蛋白浓度、滤纸酶活(FPase)、β-葡萄糖苷酶活(BG)及内切葡聚糖酶活(CMCase)比野生菌产生的分别提高了67.3%、14...Cellulose, the main composition of the vegetal cell wall, exists widely in the nature and is the most abundant reproducible bio-polymer in the earth. The degradation catalysed with cellulase is the most effective way to hydrolyze cellulose to sugars thoroughly and causes no pollution. In the production of cellulase by micro-organism, owing to low productivity of strains, low hydrolysis efficiency ...学位:工学硕士院系专业:化学化工学院化学工程与生物工程系_生物化工学号:2062007115088

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