SPR Gold Substrate Prepared by Wet Chemical Methods and the Application in Studies of Cytochrome c Modified Electrodes

Abstract

表面等离子体激元共振光谱(SurfacePlasmonResonance,SPR)是一种灵敏的光学测量方法,在生物传感,分子特异性相互作用,超薄膜界面等研究中具有重要的作用。SPR技术相关研究与应用的关键之一是制备具有SPR响应的金属基底。本论文发展了一种全水相湿化学法构建SPR金基底(SPR-Au)的方法。运用SPR仪器及电化学循环伏安法表征所制备的SPR-Au。同时以SPR-Au作为分子自组装的基片,以11-巯基十一烷酸和11-巯基十一烷醇为混合双功能试剂,通过自组装方法制备了细胞色素c(cytc)修饰电极。研究了cytc的直接电化学行为,并用电化学SPR(EC-SPR)方法获得了cytc...Surface plasmon resonance (SPR) technique is a surface-sensitive technique based on the detection of change in refractive index (RI), which has an important significance in the fields of biosensor, biomolecular interactions, and the exceed film interphase. One of the key applications of SPR is the preparation of the metal substrates. The present study explored a wet-chemical plating method of SPR ...学位:理学硕士院系专业:化学化工学院化学系_物理化学(含化学物理)学号:2052006115201

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