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Preparation of Folate Receptor Targeted Gold Nanoparticles and Application Research in Cancer Cells Detection

Abstract

纳米金由于具有独特的理化性质及良好的生物亲和效应,不仅在催化、自组装、微电子、传感器等领域表现出广阔的应用前景,在生物医学领域,如生物标记、生物检测、药物传输等方面也有重要的潜在应用价值,因此受到越来越广泛的关注。本文采用两种不同的方式制备了叶酸受体靶向型纳米金颗粒,并探讨了它们的物理化学性质、生物相容性以及对癌细胞的靶向和检测性能。 谷胱甘肽偶联法以柠檬酸钠为还原剂,用微波水热法制备出粒径均一、分散性好的纳米金(GNPs),然后纳米金与谷胱甘肽(GSH)的巯基作用,形成GSH修饰的纳米金(GSH-GNPs)。并以GSH为偶联剂,通过叶酸的氨基与GSH的羧基反应,制备出叶酸修饰的纳米金颗粒(...Gold nanoparticles (GNPs) have many unusual chemical and physical properties such as unique optical effects, novel electrical effects and excellent biological affinity. Gold nanoparticles not only are widely used in the catalysis, self-assembly, microelectronics, sensors fields, but also show broad prospects in the biomedical field, such as biological label, nucleic acid detection, gene amplificat...学位:理学硕士院系专业:材料学院生物材料系_☆生物医学工程学号:2452007115257

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