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Gene Cloning and Heterologous expression of xylanase from Hypocrea orientalis EU7-22

Abstract

木聚糖是一种多聚五碳糖,是半纤维素的主要成份,广泛的分布于植物细胞壁中,是除纤维素外自然界中最为丰富的可再生资源多糖,约占植物生物质总量的15%~35%。木聚糖主链通过β-1,4-糖苷键将木糖残基连接而成,侧链上带有不同的取代基。由于木聚糖的复杂结构,它的完全降解需要多步酶促反应完成。内切β-1,4-木聚糖酶(EC3.2.1.8)是最重要的酶之一,作用于木聚糖主链内β-1,4-糖苷键,生成木寡糖和木糖。由于木聚糖酶具有广泛的工业应用前景,已成为国内外科研的热点,不同微生物来源的木聚糖酶基因已被克隆并进行异源表达。为获得优质的低聚木糖资源,就必须获得性状优良的产木聚糖酶工程菌株。 本文以本实验...Xylan is a kind of polysaccharides, which is widely distributed in plants cell wall. In addition to cellulose, xylan is the most abundant renewable polysaccharides in nature. Accounting for plant biomass wet weight of 15% to 35%. The main chain of xylan is composed of D-xylose with β-1,4-glycoside linkage and the branch chain contains different short substituents. Due to xylan complex structure, i...学位:理学硕士院系专业:能源研究院_能源化学学号:3242010115210

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