目的：观察和研究电压门控钾离子通道Kv1.3特异性阻滞剂rMargatoxin（MgTX）和海葵毒素(Stichodactylahelianthustoxin，SHK)对人骨肉瘤MG63细胞增殖、凋亡、细胞分裂周期、细胞迁移和侵袭能力的影响，并初步探讨其分子作用机制。 方法：体外细胞培养法培养人骨肉瘤MG63细胞株，分别用特异性Kv1.3钾离子通道阻滞剂rMargatoxin和海葵毒素(SHK)阻滞骨肉瘤MG63细胞的Kv1.3钾离子通道，采用MTT法检测不同浓度rMargatoxin和SHK对细胞增殖的影响；流式细胞术(FCM)检测其对细胞凋亡和细胞周期的影响；划痕实验检测其对MG63细胞...Objective: Many evidences have shown that voltage-gated potassium channels Kv1.3 plays an important role in the growth and development of tumor. However, the participation of Kv1.3 in human osterosarcoma has not been well documented. In our study, we explored the effects of Kv1.3 blockers on the proliferation,apoptosis,cell cycle, migration and invasion in human osterosarcoma MG63 cells.We also ex...学位：医学硕士院系专业：医学院_外科学学号：2452010115329
