National High Technology and Development of China (863 Project) [2012AA022604]; National Natural Science Foundation of China [20975021, 21175023, 81171668, 21275028]; Fujian Provincial University-Industry Cooperation Science and Technology Major Program [2010Y4003]; Foundation of Fujian Key Laboratory of Hematology [2009J1004]; Scientific Research Major Program of Fujian Medical University [09ZD013]; Natural Science Foundation of Fujian Province of China [2010J06011, 2012J01428]; Medical innovation subject of Fujian Province of China [2012-CX-30]; Science and Technology Project of Fuzhou City of China [2011-S-67-3]A highly sensitive and selective method for amplified electrochemical detection for hairpin-stem-loop structured target sequences was developed based on the temperature regulation of DNA hybrids on a sandwich-type electrochemical DNA sensor. Multistep hybridization was applied to promote the hybridization efficiency of each section of sandwich structure. The results showed that both multistep and temperature-controlling hybridization techniques were both especially made to fabricate the sensor for the tendency of internal hybridization of target gene sequences. This strategy provides significantly enhanced hybridization efficiency and sequence specificity of electrochemical detection
