TOULOUSE3-BU Sciences (315552104) / SudocSudocFranceF

Chen, Xiaoyuan

Huang, Xinglu

Liu, Dingbin

Niu, Gang

Walker, A. R. Hight

Wang, He-Fang

Wang, Zhantong

Wang, Zhongliang

Yang, Jie

陈小元

Ultrasensitive
and quantitative detection of cancer biomarkers
is an unmet challenge because of their ultralow concentrations in
clinical samples. Although gold nanoparticle (AuNP)-based immunoassays
offer high sensitivity, they were unable to quantitatively detect
targets of interest most likely due to their very narrow linear ranges.
This article describes a quantitative colorimetric immunoassay based
on glucose oxidase (GOx)-catalyzed growth of 5 nm AuNPs that can detect
cancer biomarkers from attomolar to picomolar levels. In addition,
the limit of detection (LOD) of prostate-specific antigen (PSA) of
this approach (93 aM) exceeds that of commercial enzyme-linked immunosorbent
assay (ELISA) (6.3 pM) by more than 4 orders of magnitude. The emergence
of red or purple color based on enzyme-catalyzed growth of 5 nm AuNPs
in the presence of target antigen is particularly suitable for point-of-care
(POC) diagnostics in both resource-rich and resource-limited settings

Dingbin Liu (1320243)

Jie Yang (121778)

He-Fang Wang (1322805)

Zhongliang Wang (566681)

Xinglu Huang (1306614)

Zhantong Wang (1322808)

Gang Niu (133768)

A. R. Hight Walker (1322802)

Xiaoyuan Chen (322516)

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Glucose Oxidase-Catalyzed Growth of Gold Nanoparticles
Enables Quantitative Detection of Attomolar Cancer Biomarkers

Fundamental Research Funds for Central Universities (China); Intramural Research Program (IRP) of the National Institute of Biomedical Imaging and Bioengineering, National Institutes of HealthUltrasensitive and quantitative detection of cancer biomarkers is an unmet challenge because of their ultralow concentrations in clinical samples. Although gold nanoparticle (AuNP)-based immunoassays offer high sensitivity, they were unable to quantitatively detect targets of interest most likely due to their very narrow linear ranges. This article describes a quantitative colorimetric immunoassay based on glucose oxidase (GOx)-catalyzed growth of 5 nm AuNPs that can detect cancer biomarkers from attomolar to picomolar levels. In addition, the limit of detection (LOD) of prostate-specific antigen (PSA) of this approach (93 aM) exceeds that of commercial enzyme-linked immunosorbent assay (ELISA) (6.3 pM) by more than 4 orders of magnitude. The emergence of red or purple color based on enzyme-catalyzed growth of 5 nm AuNPs in the presence of target antigen is particularly suitable for point-of-care (POC) diagnostics in both resource-rich and resource-limited settings

Xiamen University Institutional Repository

Glucose Oxidase-Catalyzed Growth of Gold Nanoparticles Enables Quantitative Detection of Attomolar Cancer Biomarkers

https://figshare.com/articles/Glucose_Oxidase_Catalyzed_Growth_of_Gold_Nanoparticles_Enables_Quantitative_Detection_of_Attomolar_Cancer_Biomarkers/2034534

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Xiamen University Institutional Repository