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unknown
HIV-1 gp160截短蛋白在酿酒酵母中的表达
Authors
何芳萍
周正
+7 more
夏宁邵
张军
张毅
李少伟
李庶甘
杨坤宇
林庆山
Publication date
30 August 2011
Publisher
Abstract
利用PCR技术从pNL-43上扩增出截短的编码gp160蛋白的基因片断,克隆到酿酒酵母表达载体YEpFLAG-1上构建表达质粒YEp-gp160Δ12,电转化到酿酒酵母中,用缺色氨酸的SC培养基筛选出阳性克隆,重组子经YP培养基诱导后进行全菌蛋白的SDS-PAGE和Western Blotting分析,筛选出高表达菌株.纯化后的重组gp160Δ12(rgp160Δ12)蛋白经ELISA鉴定显示具有良好的生物活性
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Last time updated on 16/06/2016