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基因Ⅰ、Ⅳ型戊型肝炎病毒高灵敏度通用引物的设计和初步应用
Authors
夏宁邵
张军
+3 more
李少伟
葛胜祥
郭清顺
Publication date
20 May 2005
Publisher
Abstract
设计针对国内流行的戊型肝炎病毒(HEV)基因Ⅰ、Ⅳ型,在这两型序列的保守区域设计了一套RT-PCR引物———E引物,并将E引物与目前较常用的3对通用引物(Meng、ConORF1和ConORF2引物)比较了检测基因Ⅰ、Ⅳ型HEV的灵敏度。对基因Ⅰ型HEV,E引物能检出的稀释度为105,参考引物能检出的稀释度为102~104;对基因Ⅳ型HEV,E引物能检出的稀释度为102,参考引物能检出的稀释度为101~102。在17份HEV-IgM阳性血清中,E引物检出5份,检出率为29.4%;参考引物只能检出1份或2份,检出率最高为11.8%。E引物在33份HEV-IgM阳性的隐性感染血清中检出6份,阳性率18.2%;在79份HEV-IgM阳性的临床肝炎血清中检出36份,阳性率45.6%。以上结果初步表明,对于在国内流行的基因Ⅰ、Ⅳ型HEV,E引物的检测灵敏度要高于目前常用的通用引物
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Last time updated on 16/06/2016