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绿色荧光蛋白基因在大黄鱼体内的表达
Authors
周化民
王军
苏永全
鄢庆枇
Publication date
10 April 2002
Publisher
Abstract
以绿色荧光蛋白基因为报告基因 ,以pIRESneo为载体质粒 ,制备含有报告基因的质粒 ,以肌肉注射的方式导入大黄鱼体内 ,每隔一周用荧光显微镜观察绿色荧光蛋白基因在鱼体不同部位的表达情况 .结果表明 ,绿色荧光蛋白基因可以在大黄鱼体内得到表达 ,表达时间高达 2 8d以上 .绿色荧光蛋白基因不仅可以在肌肉注射部位组织细胞得到表达 ,而且在其它部位的肌肉、肝脏、肾脏和心脏等组织中也有表达 ,但表达水平低于肌肉注射部位组
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Last time updated on 16/06/2016