CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
unknown
铜铁试剂对菜青虫多酚氧化酶的抑制作用
Authors
柯莉娜
王勤
+3 more
罗万春
薛超彬
陈清西
Publication date
15 April 2005
Publisher
Abstract
以菜青虫 Pieris rapae (L.) 为试材, 冰浴匀浆, 4℃下 6 403 ×g 离心, 取上清液, 经 35%饱和度 (NH4)2SO4 沉淀,Sephadex G-100凝胶过滤柱层析等分离步骤, 获得部分纯化的菜青虫多酚氧化酶制剂。研究不同浓度铜离子及铜铁试剂对该酶的影响, 结果表明: Cu2+在0~0.100 mmol/L范围内对酶活力表现激活作用; 浓度大于0.125 mmol/L时表现抑制作用, 其IC50为0.651±0.022 mmol/L; 铜铁试剂对该酶抑制作用的IC50为0.100±0.012 mmol/L。抑制动力学研究结果表明: 铜铁试剂对该酶表现为可逆抑制效应, 为竞争性抑制类型, 其抑制常数Ki 为0.076±0.013 mmol/L
Similar works
Full text
Open in the Core reader
Download PDF
Available Versions
Xiamen University Institutional Repository
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:dspace.xmu.edu.cn:2288/199...
Last time updated on 16/06/2016