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丙氨酰谷氨酰胺二肽对人脐静脉内皮细胞ECV304缺氧缺糖损伤的保护作用
Authors
吴振
唐果
+4 more
怀文辉
王字玲
赵敬湘
赵玉芬
Publication date
15 February 2009
Publisher
Abstract
目的观察丙氨酰谷氨酰胺二肽(丙谷二肽)对人脐静脉内皮细胞ECV304缺氧缺糖损伤的保护作用,并探讨其可能的作用机制。方法在以低氧低糖培养人脐静脉内皮细胞ECV304为细胞损伤模型的基础上,以噻唑蓝(MTT)比色法优化丙谷二肽的最佳作用浓度,显微镜观察细胞形态变化,流式细胞术检测线粒体膜电位。自动生化分析仪测定乳酸脱氢酶(LDH)活性,比色法检测谷胱甘肽(GSH)、丙二醛(MDA)的浓度,RT-PCR方法检测细胞内肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、热休克蛋白70(HSP70)、葡萄糖调节蛋白78(GRP78)和缺氧诱导因子-1α(HIF-1α)mRNA的表达。结果丙谷二肽能够使细胞在缺氧缺糖应激下存活率增加,线粒体损伤减轻,LDH分泌降低,GSH产生增加,HSP70和HIF-1αmRNA的表达增加。结论丙谷二肽对细胞缺氧缺糖损伤有明显的保护作用,这种保护作用可能与保护线粒体、维持细胞膜结构完整、上调细胞中应激基因HSP70和HIF-1α的表达有关
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Last time updated on 16/06/2016