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微柱离心-HPLC法测定六甲蜜胺脂质体包封率
Authors
曹金娜
王秀敏
邓英杰
高晓非
Publication date
28 February 2009
Publisher
Abstract
目的:对六甲蜜胺脂质体进行质量评价,建立六甲蜜胺脂质体包封率的测定方法。方法:采用(凝胶)微柱离心法分离游离药物与脂质体;利用流动相:甲醇-乙腈-水(50∶35∶15);柱温:30℃;流速:0.8 mL·min-1;紫外检测波长:228 nm的HPLC条件测定六甲蜜胺的含量,最终计算得包封率。结果:微柱离心法能够将100%的空白脂质体和小于3.5%的游离药物洗脱下来,游离药物与脂质体可被分离,游离药物基本上被截留于微柱中。在本法选择的色谱条件下,六甲蜜胺得到良好的分离,辅料不干扰测定,六甲蜜胺在2.0~40.0 mg·L-1内线性关系良好(r=0.9996),日内和日间RSD均<2.0%(n=5),加样回收率在97.9%~98.5%之间(RSD<2.0%,n=5)。用该方法测得3份六甲蜜胺脂质体的包封率在82.49%~86.56%之间(RSD均<2.0%,n=3)。结论:本方法可用于测定六甲蜜胺脂质体包封率
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Last time updated on 16/06/2016