通信作者:[email protected][中文文摘]以一株分离自福建省武夷山自然保护区的喜树内生真菌SXZ-19为研究对象,通过固体平板发酵培养对菌体的化学成分进行了研究.从10L发酵菌体提取物中分离得到8个单体化合物,通过核磁共振以及质谱数据鉴定了化合物1～8的化学结构,分别为:2个线性呋喃聚酮类化合物(1～2);2种真菌毒素(7～8),交链孢酚单甲醚(alternariol monomethyl ether,AME)和交链孢酚(alternariol,AOH);4个洛伐他丁类似物oblongolides D(3),P(4),H(5)和V(6),其中化合物4和5互为差向异构体,为从同一菌株中分离得到oblongolides的差向异构体.[英文文摘]Endophytic microorganisms are a significant reservoir of genetic diversity,and an important source for the discovery of novel bioactive secondary metabolites.In the course of our ongoing efforts to discover potential anticancer agents from endophytic fungi,eight natural compounds,including two linear furanopolyketides (1-2),two Alternariatoxins (7-8),namely alternariol monomethyl ether (AME)and alternariol (AOH),and four lovastatin analogues (oblongolides D (3),P (4),H (5)and V (6)),were isolated from the endophytic fungal strain Diaporthe sp.SXZ-19of Camptotheca acuminate.The strain was collected from National Conservation District Wuyi in Fujian Province,China,and deposited in the laboratory of Microbial Pharmaceutical ngineering,Xiamen University,China.The chemical structure of these compounds were elucidated by spectroscopic analyses including1 H and 13 C-NMR,2D NMR(HMQC,HMBC,1 H-1 H-COSY and NOESY)and ESI-MS.Of these compounds,oblongolides P (4)and H (5)are epimers,which was the first time to report the isolation of two epimers of oblongolides from one strain．科技部国际科技合作项目(2007DFA30970);中央高校基本科研业务费专项(2010121092,2011121037
