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unknown
Optimizing recovery and cloning of positive bands in AFLP analysis
Authors
Virginia Tech, Blacksburg, VA24061, USA) M.S.Saghai Maroof ( Department of Crop and Soil Environmental Science
郭小玲(新乡市环境保护局)
陈亮
马凌波
Publication date
1 August 2000
Publisher
《生物技术》编辑部
Abstract
通过将AFLP聚丙烯酰胺变性胶切小、适当曝光过量、以聚丙烯酰胺凝胶电泳代替琼脂糖电泳检测克隆片段大小的方法 ,增加了回收克隆AFLP阳性带的准确性。该方法对于从变性和非变性大page胶上回收克隆目的片段具有普遍意义
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Last time updated on 16/06/2016