从膜通量、蛋白去除率、核苷酸收率、膜污染和膜清洗等方面考察了5种不同型号的Ultra-floTM超滤膜过滤RNA酶解液的效果。实验表明3#(MWCO30 kD,CA)Ultra-floTM超滤膜同时具有高收率(96.4%)、高蛋白去除率(81.3%)、高膜通量(65.6L.m-2.h-1)、易清洗(水通量恢复99%)的优点,不仅可以替代通常使用的中空纤维膜,且通量大、污染小、易清洗,使该过程得到优化。The filt ration performances of enzymatic RNA degradation liquid wit h five different Ult ra2flo TM ult ra2
filt ration membranes have been st udied by comparing flux , protein rejection , nucleotide yield , membrane foul2
ing and membrane cleaning. The result s show t hat 3 # Ult ra2flo TM ult rafilt ration membrane (MWCO 30kD) was
t he optimum , having a high nucleotide yield (96. 4 %) , high protein rejection (81. 3 %) , high flux (65. 6L ·m- 1
·h - 1 ) , high recovery ratio of water flux (99 %) . This characteristic of high flux , low fouling and easy cleaning
leads us to conclude t hat 3 # Ult ra2flo TM ult rafilt ration membrane was a good candidate for replacing t he com2
monly used hollow fiber membrane to achieve an optimized filt ration of enzymatic RNA degradation liquid.福建省科技重点资助项目(2000-I-015);福建省青年科技人才创新资助项目(2001J065)
