[中文文摘]采用分光光度法测定蜜环菌 (Armillaria mellea)胞外漆酶 (L accase)的活力 ,分别以 2 ,2’-连氮 -二 (3-乙基苯并噻唑 - 6 -磺酸 ) (简称 ABTS)、丁香醛连氮、愈创木酚和邻联甲苯胺为底物 ,比较了这4种底物对漆酸的敏感性 ,探讨了酶反应时间、温度、缓冲液及其 p H值和底物与酶的用量等条件对漆酶催化反应的影响 .实验结果表明 ,适宜的测酶条件可以确保漆酶活力的测定快速而准确。[英文文摘]Spectrophotometric determination was taken to determine extracellular laccase activity from Armiillaria mellea . ABTS, syringaldazine, guaiacol and tolidine was used as the substrate for laccase respectively. The effects of time, temperature, buffer and its pH, and the amount of substrate and enzyme on laccase catalytic reaction were studied. The experimental results showed that suitable determination could ensure rapid and accurate determination of laccase activity.安徽省科委自然科学基金资助项目(98212411
