集中使用寡核苷酸、肽核酸和细胞凝素3类探针对来自东海和厦门海域的现场赤潮样品进行了检测,尝试鉴定识别自然水样中有害的赤潮原因种塔玛亚历山大藻,微小原甲藻和纤小裸甲藻,建立和优化了这些探针的检测方法和样品处理程序。结果表明,在东海和厦门海域的赤潮样品中均成功地检出了塔玛亚历山大藻的分布情况,各探针的检测效率为DBA>Tama28S>Tama5S;在东海和厦门海域的赤潮样品中,也成功地检测出了微小原甲藻,各探针的检测效率为:ConA>PM18S02>PM28S02;在厦门海域的赤潮水样中检出了纤小裸甲藻,各探针的检测效率为:WGA>PNATP28S01>TP18S02>TP28S01。各探针检测结果与相关文献的报道吻合较好。比较这3类探针的特异性,其中以PNA探针为最好,其次为DNA;lectin探针的特异性相对较弱。
【英文摘要】 Molecular probes are useful to separate closely related harmful algal bloom species. Moreover, they can rapidly and clearly differentiate non-toxic from toxic algae and to monitor the development of algal blooms in coastal waters. There are three kinds of molecular probes which can be used for identification of HAB species, such as lectin, DNA and peptide nucleotide acid(PNA) probe. These probes are relatively new, and have higher sensitivity, veracity and specificity than traditional taxonomic techniques. ...国家“九七三”重点基础研究发展规划(CEOHAB2001CB409704);; 中国博士后基金(20060400854);; 农业部淡水鱼类种质资源与生物技术重点实验室开放课题(LFB20070611);; 厦门市创新科技基金(3502Z20041059);; 教育部博士点基金(20070504076
