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The Effects of Physical and Chemical Conditions on Forming Mycelial Pellet of Phanerochaete chrysosporium and Biosorption of Lead

Abstract

[中文文摘]为了探讨影响微生物菌丝球生长的物理化学因素和控制菌丝球大小的规律以及微生物吸附重金属的效果,对黄孢展齿革菌(Phanerochaetechrysosporium)呈球状体生长和用此菌丝球吸附水溶液中的Pb2+进行了研究.实验结果表明,在培养液pH值为4.5,孢子悬液浓度为106个/ml,表面活性剂吐温80的浓度为0.1%,高碳氮和摇床转速为150r/min的条件下,于39℃下培养3d,形成直径在1.5—1.7mm范围内的菌丝球,光滑均匀,具有一定机械强度,对Pb2+的吸附能力最强.用0.2mol/L的NaOH溶液处理该菌丝球,对25mg/L的铅溶液的吸附率达到了95%以上,表明用该菌丝球吸附水溶液中的Pb2+是可行的.[英文文摘]The research on the growth of Phanerochaete chrysosporium in the form of pellet was made in this paper to examine the effects of physical and chemical conditions on the formation of mycelial pellets.The experimental results showed that the microorganism pellets of smooth surface,even size(diameter)and good mechanical properties could be obtained at the follwoing culture conditions:pH4 5,10 6 spores/ml in inoculum,01% Tween 80 in medium,high C/N and on the shaker of 150r/min for 3d.Using P. ch ry sosp orium m ycelial pellet s in the bio sorption of Pb2+ from aqueou s solution, the up take reached the maximum when the diam eter of pellet w as in the range of 115—117mm. W hen the pellet s w ere p ret reated by bo iling in 012mo löL N aOH fo r 40m in, the Pb2+ up take w as far h igher than the pellets without pret reatm en t, and the removal efficiency could reach above 95%. The research results showed that the removal of Pb2+ from aqueous solution by P. chrysosp oriumm ycelial pellets was practical.国家教委留学回国人员科研启动基

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