We investigated the segregation of an intracellular microsporidian parasite during host cell division. A time-course
experiment was carried out to examine the distribution of parasites relative to host chromosomal DNA via light and
electron microscopy. Fluorescent light microscopy and EM studies showed that the parasite lay in the perinuclear zone
of the host cell during interphase and segregated to daughter cells at mitosis. At metaphase, the parasite was frequently
closely associated with host microtubules and mitochondria. Electron-dense bridges were observed between the parasites
and the host microtubules and also between host mitochondria and microtubules. The study suggests that both the parasite
and the host cell organelles segregate in association with spindle microtubules
