OGATA, Norichika

Sakamoto, Hironori

SASAKI, Tetsuhiko

English

Sydney eScholarship

> < Epigenetics of brain development in workers of                              the European honeybee, Apis mellifera  Hironori Sakamoto1，Norichika Ogata2，Tetsuhiko SASAKI1（1Tamagawa Univ.  2Japan Bio-data）  ○ Introduction Materials and Methods Compare genome-wide methylation pattern of brains between nurses and foragers                   by  bisulfite sequencing using a next-generation sequencer  Next generation sequencer can read more than 6 Gb (60, 000, 000, 000 bases) /run    Bisulfite treatment converts         unmethylated C to U DNA extraction Bisulfite conversion Results and Conclusions 5’   GAATUGAC*GA   3’ CH3 5’   GAATCGAC*GA   3’ CH3  DNA methylation occurs at           C of CG (GpG) dinucleotides   Queen Worker Learning ability increases with aging  (nurse <<  forager )  (Ichikawa and Sasaki 2003) Worker honeybees change their task with aging Young bees…nurses (inside nest, feed larvae) Old bees…foragers (outside nest, collect honey) Does epigenetic mechanism regulate brain function ?            We focused on DNA methylation   (safe to risky) 1. < 1% of CpG sites were methylated.  4. Methylation-patterns were brain-specific.             binomial test, FDR<0.05 Exon Intron Others 7,137,460 （99.5%） 7,711,210 （99.3%） 42,172 （0.5%） 55,784 （0.7%） methylated Not methylated Brain(NB) 42.172 Brain (FB) 55,784 Hypopharyngeal Glands (NH) 38,688 Hypopharyngeal Glands(FH) 20,949 Total 2986 (NB>NH 2965, NB<NH 21) Total 620 (FB>FH 562, FB<FH 58) Total 774 (NB>FB 419,NB<FB 355) Total 120 (NH>FH 19,NH<FH 101) 2. Differently methylated CpG sites                              were exon-located. 359 （85.6%） 303 （85.3%） 42 （10.0%） 46 （13.0%） 18 （4.4%） 6 （1.7%） Including 275 genes Including 225 genes 3. Differently methylated genes               regulated gene expression       Predicted function remarkable genes histone modification    Sir-2,  Hdac3 DEAD-box helicase      LOC726768 chromatin remodeling   Iswi   neural development     big brain            

Epigenetics of brain development in workers of the European honeybee, Apis mellifera.

https://ses.library.usyd.edu.au/bitstream/2123/11361/3/Poster162.pdf

Epigenetics of brain development in workers of the European honeybee, Apis mellifera.

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