Aoyama, Hiroaki

Kumara, Rohitha

Saitoh, Seikoh

Tokuda, Gaku

English

Sydney eScholarship

  Transcriptome analysis on physiological roles in the mixed segment        Termites e. cockroaches) decomposition. Termites ). Nasutitermes higher termites segment (P some physiological not been mixed segment       comprise a group of social insects usually classified as a part of the order Blattodea (i. and are ecologically very important due to their ability of lignocellulose  are divided into two groups namely, lower termites and higher termites (i.e. the family Termitidae takasagoensis is a wood-feeding higher termite. A remarkable feature of the gut in  is the presence of so-called “mixed segment” between the midgut and first proctodeal 1). The mixed segment consists of the mesenteric and proctodeal epithelial tissues. Although  characteristics of the mixed segment have recently been clarified, yet the function has  fully elucidated. Here, we carried out 454 pyrosequencing to profile the transcriptome of the  and compared with those of the midgut and P1 to understand the function of the mixed segment.1. Introduction P062 2. Aim of the study  3. Experimental procedure  3.1 Data annotation process  4. Results We propose that V-ATPase genes play an important role in the mixed segment.  Further, it may be responsible for an ion transport and catabolism  1.1 Digestive system in N.takasagoensis   Transcriptome analysis To obtain information on the  genes and metabolic pathways  working in the mixed segment (Bignell et al. 1983) (Köhler et al. 2012) Model proposed by Bignell and  co-workers Axial profiles of pH along the gut of N. corniger, measured at the gut center. pH sharply  increased in the MS, and when it  comes to P1, it shows the highest pH value.     There is an active potassium ion flux across MS tissue to the lumen from haemolymph.  1.2 Previous findings 4.1.Overall statistics    Midgut (MD), mixed segment (MS), and P1 tissues were collected from worker termites  cDNA libraries were prepared for the 454 GS junior sequencing Sequencing reads were assembled on Newbler ver. 2.7p Generated isotigs (transcripts) were annotated 4.2 Comparative analysis What is function of the mixed segment??  Comparative analysis and Pfam annotation revealed the differences in gene compositions in 3 tissues. 4.3 Pfam annotation 4.4 Level 2 Gene Ontology analysis Glycolysis TCA cycle Oxidative phosphorylation  Phagosome Lysosome Synaptic vesicle cycle  4.5  Highly abundant KEGG pathways         identified in mixed segment (Relative         abundance of reads)  4.6 Relative mRNA expression levels of V-ATPase subunits in 3 tissues V- ATPase from laval Manduca sexta midgut Ion influx (K+) Biological process Cellular component Molecular function Relative abundance of reads ATP ADP + PiH+membrane peripheral Based on the present/absent information per gene for libraries 010203040MD MS P1Relative Expression Levelsaab0246MD MS P1Relative Expression Levelsabc0100200300400MD MS P1Relative Expression Levelsa cb16KDa-proteolipid subunit010203040MD MS P1Relative Expression Levelsbaa02468MD MS P1Relative Expression Levelsabc0246810MD MS P1Relative Expression Levelsaba010203040MD MS P1Relative Expression Levelsaab0100200300400MD MS P1Relative Expression Levelsaab02468MD MS P1Relative Expression Levelsabc050100150200MD MS P1Relative Expression levelsaabd1-AC39 subunit A  subunitE subunitD subunitC subunitB  subunitH subunitF  subunit G subunit(Salivary glands) Foregut FG Paunch P3 First proctodeal segment P1 Mixed Segment MS Midgut MD dbCAN database describes  the carbohydrate active enzymes KEGG dealing with  genomic information and metabolic pathways isotigs GO provides an ontology  of defined terms representing   gene products attributes  across all species Pfam is a database  of protein families Significant differences of  functional variations in 3 tissues (Wieczorek et al. 2003) Dominant expressions of V-ATPase subunits in MS were confirmed with qPCR analyses.  Highly expressed Pfam domains  010203040MD MS P1Relative Expression Levelsaab0246MD MS P1Relative Expression Levelsabc0100200300400MD MS P1Relative Expression Levelsa cb16KDa-proteolipid subunit010203040MD MS P1Relative Expression Levelsbaa02468MD MS P1Relative Expression Levelsabc0246810MD MS P1Relative Expression Levelsaba010203040MD MS P1Relative Expression Levelsaab0100200300400MD MS P1Relative Expression Levelsaab02468MD MS P1Relative Expression Levelsabc050100150200MD MS P1Relative Expression levelsaabd1-AC39 subunit A  subunitE subunitD subunitC subunitB  subunitH subunitF  subunit G subunitRicin-type beta-trefoil lectin domain ATP synthase subunit c  Actin  Several domains pertaining to V-ATPases  Haemolymph juvenile hormone binding     protein   Insect allergen related repeat, nitrile-    specifier detoxification   Glycosyl hydrolase family 9  Trypsin  Reverse transcriptase   Actin  Myosin head   Myosin tail  MD MS P1 Tropical Biosphere Research Centre, University of the Ryukyus, Okinawa  Japan. Rohitha P. Kumara, Seikoh Saitoh, Hiroaki Aoyama, Gaku Tokuda Description MD MS P1  Putative host genomic mRNA 76197 45283 24603  Isotigs (transcripts) 4813 4563 3629  Isogroups (unigenes) 3821 3607 2823  Not considered for analysis 78517 70945 89610  Total number of reads 154714 116228 114213 

Transcriptomic analysis on physiological roles in the mixed segment

https://ses.library.usyd.edu.au/bitstream/2123/11268/4/Poster062.pdf

Transcriptomic analysis on physiological roles in the mixed segment

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