Center for Antibody Production Rijeka (CAPRI) is a part of the Center for Proteomics devoted to
mouse monoclonal antibody production. The Center for Proteomics is a department at the University of Rijeka
Faculty of Medicine that is to a large extent self-sustainable since it employs scientists, administrative and
technical staff and covers all its research costs from competitive international grants which makes it a unique
department in the Croatian academia. One of the objectives of the Center for Proteomics is to put emphasis on
applied R&D and utilization of the existing large collection of antibodies and antibody-based products, both
suitable for further commercial development

Lenac Roviš, Tihana

Center for Antibody Production Rijeka (CAPRI) is a part of the Center for Proteomics devoted to mouse monoclonal antibody production. The Center for Proteomics is a department at the University of Rijeka Faculty of Medicine that is to a large extent self-sustainable since it employs scientists, administrative and technical staff and covers all its research costs from competitive international grants which makes it a unique department in the Croatian academia. One of the objectives of the Center for Proteomics is to put emphasis on applied R&D and utilization of the existing large collection of antibodies and antibody-based products, both suitable for further commercial development

Repository of the University of Rijeka, Faculty of Medicine

294CENTER FOR ANTIBODY PRODUCTION RIJEKA (CAPRI)- TOWARDS A NEW RESOURCE FOR ANTIBODY-BASED TOOLSTihana Lenac RovišUniversity of Rijeka Faculty of Medicine, Center for Proteomics, Brace Branchetta 20, 51000 Rijeka, CroatiaAbstract — Center for Antibody Production Rijeka (CAPRI) is a part of the Center for Proteomics devoted to mouse monoclonal antibody production. The Center for Proteomics is a department at the University of Rijeka Faculty of Medicine that is to a large extent self-sustainable since it employs scientists, administrative and technical staff and covers all its research costs from competitive international grants which makes it a unique department in the Croatian academia. One of the objectives of the Center for Proteomics is to put emphasis on applied R&D and utilization of the existing large collection of antibodies and antibody-based products, both suitable for further commercial development.Index Terms — antibodies, antibody based recombinant proteins, cytomegalovirus, varicella zoster virus, lymphocyte receptors_____________________________________________________________________ 1 BACKGROUNDCAPRI, as a part of the Center for Proteomics, possesses an excellent collection of monoclonal antibodies (mAbs) for research purposes. A major part of this unique collection can be used to investigate the functions of viruses that are widely spread among the world’s population. CAPRI has also generated a set of mAbs against surface receptors on human and mouse lymphocytes. These mAbs are fully characterised and ready for market entry. In addition, CAPRI has several research projects based on its mAbs, with the potential to develop new and innovative products.S. Passamonti, S. Gustincich, T. Lah Turnšek, B. Peterlin, R. Pišot, P. Storici (Eds.) CONFERENCE PROCEEDINGS with an analysis of innovation management and knowledge transfer potential for a smart specialization strategy ISBN 978-88-8303-572-2 / e-ISBN  978-88-8303-573-9. EUT, 2014.CROSS-BORDER ITALY-SLOVENIA BIOMEDICAL RESEARCH:ARE WE READY FOR HORIZON 2020?2952 OBJECTIVESUtilise the generated antibodies for research, diagnostic and therapeutic applications.Increase the visibility and awareness of business stakeholders about science-business interaction opportunities.3 APPROACH & METHODSGeneral approachCAPRI was established with the focus on production and purification of recombinant proteins and mAbs against viral and cellular proteins. The goal of the CAPRI’s spin-off project is to commercialise the available research products and attract grant funds for new and existing products from CAPRI’s mAb portfolio.Methods1. Custom mAbs/hybridoma development  Protein (immunogen) production via His-tag and GST-tag purification from E. Coli and Fc-fusion protein production in eukaryotic cells2. Construction of recombinant proteins (potential therapeutics based on mAbs)3. Construction of new protein and RNA expression vectors4. In vivo  methods (state of the art animal facility with the impressive collection of genetically modified mice with alterations in various aspects of immune response)4 RESULTSFigure 1: Varicella proteins visualized in the cell culture and in the human skin and ganglium using our unique mAbs296Figure 2: CAPRI’s mAb bank5 POTENTIAL NEW PRODUCTS & SERVICES1. The collection of mAbs against ~100 target proteins, such as immune cell receptors, their ligands and viral proteins. For most of these protein targets there are no other antibodies available anywhere in the world. 2. Innovative recombinant immunotherapeutics3. Immunotoxin for treatment of glioblastoma multiforme4. Immunotherapeutic for bridging  the  innate  and  the  specific  antiviral  response5. New strong viral promotorCenter for Proteomics has produced, purified and packed its mAbs according to the standardised protocols, generated a web platform offering its mAb collection and supporting each mAb with the corresponding data sheet file.The database is available at : http://products.capri.com.hr/2976 CURRENT COLLABORATIONSIn the mAb development Center for Proteomics currently collaborates with the numerous research institutions:1. University of Alabama, Birmingham, USA 2. Medical University, Innsbruck, Austria3. Max von Pettenkofer Institute, LMU, Munich, Germany4. National Institutes of Health (NIH), USA 5. Heinrich-Heine University, Duesseldorf, Germany6. Medical School, Hannover, Germany7. Laboratory of Immunology, NIAID, Maryland, USA8. Ruhr-Universitat Bochum, 9. Medizinisches Proteom-Center, Germany10. Robert Koch-Institute, Berlin, Germany11. The Rudjer Boskovic Institute, Zagreb, Croatia7 CONTACT OR COLLABORATIONS NEEDEDCenter for Proteomics is interested in future collaboration with companies/institutes or consultants with extensive experience in GMP and SOP practices in the field of large scale biopharmaceutical production.8 COMMUNICATION TOOLSSelected relevant publications  2013:1. Lenac Rovis T et al. A comprehensive analysis of varicella zoster virus proteins using a new monoclonal antibody collection. Journal of Virology, 20132. Juranic Lisnic V et al. Dual Analysis of the Murine Cytomegalovirus and Host Cell Transcriptomes Reveal New Aspects of the Virus-Host Cell Interface. PLoS Pathogens, 20133. Trsan T et al. Superior induction and maintenance of protective CD8 T cells in mice infected with MCMV vector expressing RAE-1γ. PNAS, 2013More on current research projects can be found on: http://www.capri.com.hrContact details:E-mail: info@capri.com.hrTelephone: +385 51 651 2602989 FUNDS NEEDEDForecast on the basis of three years9.1.  For applied research on mAb bank exploitation  (solutions for real-world problems): 100.000 €9.2   For  ongoing basic research on recombinant immunotherapeutics     (investigation of biological mechanisms): 350.000 €9.3  For  subsequent pilot & demonstrator activities (to develop a prototype):    50.000 €10 CONCLUSIONCAPRI will continue developing its antibody portfolio using the funds granted from various sources. The diversification of antibody commercialization processes offers plenty of opportunities for collaborative projects with academia and innovative SMEs, including licensing opportunities. Please contact us in case you are interested in the presented antibody bank or antibody-based compounds. We also offer a full-service development of new and reliable custom-made monoclonal antibodies.ACKNOWLEDGEMENTThis work was supported by the “Regional Competitiveness” operational programme 2007-2013; project “Becoming entrepreneurial: Knowledge transfer from the University of Rijeka Faculty of Medicine to the biotechnology business sector” (IPA2007/HR/16IPO/001-040517).ReferencesLenac Rovis T et al. A comprehensive analysis of varicella zoster virus proteins using a new monoclonal antibody collection. Journal of Virology, 2013Juranic Lisnic V et al. Dual Analysis of the Murine Cytomegalovirus and Host Cell Transcriptomes Reveal New Aspects of the Virus-Host Cell Interface. PLoS Pathogens, 2013Trsan T et al. Superior induction and maintenance of protective CD8 T cells in mice infected with MCMV vector expressing RAE-1γ. PNAS, 2013

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Center for antibody production rijeka (capri)- towards a new resource for antibody-based tools

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