Pusobeni kadmia a zinku na funkce lidskych lymfocytu in vitro.

Abstract

We tested the effect of metals sulphates added in cell cultures in different concentrations from 10-2 M to 10-10 M. Cell cultures were prepared from the whole human blood without the isolation of lymphocytes. We studied the early cell activation by the expression of the surface antigen CD69, the blast transformation of lymphocytes, changes of CD95 expression, the production of cytokines (intracellular and in the supernate) and the production of immunoglobulins. The duration of cultivation was different in XXXX methods. The analysis of the expression of surface cells antigens (lymphocytes subpopulations, CD69, CD95), blast transformation and intracellular cytokines was made with monoclonal antibodies by flow cytometry. The results of the concentration of produced cytokines were obtained by ELISA, the concentration of immunoglobulines was detected by nephelometry. The result showed more expressive differences in the test of blast transformation. There were stimulating effects of cadmium in concentrations 10-3 M, 10-4 M and 10-8 M, and zinc 10-3 M. The most suppressive effect was in concentrations 10-10 M of cadmium and 10-8 M of zinc. The highest CD69 expression was in concentration 10-4 M and 10-6 M of cadmium and 10-3 M of zinc. The kinetic study of the expression of the surface antigen CD95 (Fas receptor) showed the elevation of the percentage of positive untreated cells after 18 hours of cultivation and a very low expression after 90 hours. In cell cultures with cadmium and zinc was the CD95 expression on lymphocytes after 18 hours lower that results of untreated cells. After 90 hours of cultivation we found difference between the effect of both metals, with a great individal variability in the number of positive cells upon the influence of zinc.In the test of cytokine production there were significant differences between the groups of studied persons, patients with autoimmune diseases showed high levels of IL-6 in all cell cultures, the group of heavy smokers produced IL-10 after the stimulation with PHA. In the detected concentrations of produced cytokines and immunoglobulins (very low levels) we found high individual variability. The results obtained by our experiments show the importance of the study of the influence of metals on the functions of immune system. Our investigation shows possibilities for further research.Available from STL Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi