Produkty pokrocile glykace a produkty pokrocile oxidace proteinu a jejich klinicky vyznam.

Abstract

Pathogenesis of many diseases and their complications is connected with oxidative and carbonyl stress. Advanced glycation end products (AGEs) and advanced oxidation protein products (AOPP) are two outstanding markers of these mechanisms. The aim of the thesis was to introduce a method for determination of AGEs and AOPP and their studying in diabetology and nephrology. ELISA method for AGE-assessment was tested in our laboratory. Even repeated glycation, immunization and purification didn't result in suitable antiserum. This method is very discutable and its use for AGE-determination is becoming obsolent. A spectrofluorimetric method for AGE-determination was introduced and gel permeation chromatography with fluorescent detection was used for characterisation of fluorescent substances. AOPP were determined with a spectrophotometric method. AGEs and AOPP were examined in patients with type 1 and 2 diabetes mellitus, in hemodialyzed patients with and without diabetes mellitus, in hemodialyzed patients during dialysis, and in healthy subjects for comparison. Our studies show a useful application for determination of glycoxidation parameters -AGEs and AOPP - in the course of chronic diseases and their complications in patients with diabetes mellitus and chronic renal failure.Available from STL Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi