Contribution to the genome projects strategies.

Abstract

I have been involved in two major genomic projects, namely the Arabidopsis thaliana genome project and the European Drosophila genome project. I determined approximately 318 Kbp of the Arabidopsis thaliana sequence and approximately 250 Kbp of the Drosophila melanogaster sequence. Using the available computational tools, I identified 76 protein-coding genes and one tRNA gene within the Arabidopsis sequences and identified 17 genes with homology to known proteins within the Drosophila sequences. It was possible to assign a probable function for 42 out of 76 protein-coding genes (55%) from the Arabidopsis based on homology to known proteins. The function can be predicted for 12 out of 17 protein-coding Drosophila genes (70%). I identified 5 genes, which are potentially medically important. I also contributed to the development of techniques which can be used for deleting redundant regions of overlapping BAC clones prior to sequencing.Available from STL Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi