Bunky prezentujici antigen a jejich vyuziti v lecbe hematologickych malignit - optimalizace ziskavani a expanze dendritickych bunek.

Abstract

Anticancer immunotherapy is a strategy aimed at the induction and maintenance of immune responses against malignant cells. In the last decade, vaccines containing antigen-presenting cells (APC) loaded with tumor-associated antigens have been assessed in clinical experiments. Dendritic cells (DC) are known as the most efficient APC. Development and optimization of DC culture methods for vaccination purposes and the elaboration of a protocol for DC-based immunotherapy of multiple myeloma was the objective of this thesis. Series of experiments were performed in order to compare the yield, immunophenotype and function of DCs generated by different methods. The experimental design permitted us to use appropriate tests for pairs in the analysis of results. The generation of DCs from PBSC in two-step protocol was recommended for clinical use as the most suitable. Culture in plastic wells provides superior yields while culture in bags is preferable according to GMP. DCs alone are apparently insufficient for the production of cytolytic T-lymphocytes and non-specific stimulation with cytokines has been proposed for the future clinical trial.Available from STL Prague, CZ / NTK - National Technical LibrarySIGLECZCzech Republi