Studying the mrna-bonding centre of the ribosome on the stages of initiation and elongation of translation

Abstract

The object of investigation: the prokariotic ribosome E. coli. The work is aimed at studying the location of the matrix RNA on the ribosome in the different initiation comlexes and in the elongation ribosome using the method of the affine modification. The modification of ribosomes by the photoaffine analogs of the mRNA has been performed. Stitches mRNA-16s RNA have been analyzed by the RNA-ase H hydrolisis, the mRNA-proteins using the immunochemical method. The new method intended for the analysis of stitches mRNA-16s RNA has been developed. Specific areas 16s RNA and ribosome proteins, which are drawn nearer with mRNA on the different stages of translation, have been identified. The results have served as the base for the model of space organisation 30s subparticle for reconsiderationAvailable from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio