THE PROTEINS OF EUCARYOTES, BINDING WITH THE SINGLE-THREAD DNA (SSB-PROTEINS): THE CHARACTERISTIC AND PARTICIPATION IN REPLICATION AND REPAIR OF DNA

Abstract

The SSB-proteins have been isolated from the ascitic carcinoma of Erlich and from the grains, its properties (molecular mass, pl, coupling of the DBA with RNA, the ability of stimulating the DNA-polymerases and inhibiting the nuclease) have been studied. The method of the quantitative determination of the SSB-proteins has been developed. From the blood plasma cleaned and characterized have been the protiens, coupling with the single-thread DNA (similar ones to antibodies to the DNA), the level of which increased after the animals radiation. The prooves of participation of the SSB-proteins in replication and repair (the proportion between its level and the size of DNA of the different organisms; the intensity of replication and repair of the DNA; the connection of the SSB-proteins with the DNA in vivo and stimulation of replication and repair by them) have been obtained. The specificity of the role of the SSB-proteins in the DNA replication has been shown.Available from VNTIC / VNTIC - Scientific & Technical Information Centre of RussiaSIGLERURussian Federatio