Erprobung eines speziellen Verfahrens des biological monitoring zum Erkennen von beruflich bedingten Schaedigungen der menschlichen Erbsubstanz Schlussbericht

Abstract

Detection of primary DNA damage such as DNA strand breaks, DNA cross links or DNA adducts in peripheral mononuclear blood cells can be applied to evaluate the genotoxic hazard of workers due to chemicals in their occupational environment. We used alkaline elution of DNA and "3"2p-postlabelling of DNA adducts to detect DNA damage. In sterilization workers exposed to ethylene oxide, metal workers exposed to N-nitrosodiethanolamine, petrol-pump attendants and students exposed to formaldehyde an increase in DNA damage depending on the extent of environmental exposure could be detected. No significant difference in the extent of DNA damage was found in cabinet makers and road paving workers compared to controls without apparent genotoxic exposure. Using these methods a genotoxic hazard of workers can be evaluated very quickly and therefore these methods can be applied for a short-term control of the success of safety environment. Also the genotoxic hazard to humans due to new substances in the manufacturing process or by environmental pollution due to accidents in chemical plants can be evaluated. (orig.)SIGLEAvailable from TIB Hannover: F95B748+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman