Entwicklung einer Ultraschall-Levitations-Apparatur fuer die Probenvorbereitung in der Mikro- und Spurenanalyse

Abstract

An acoustic levitation device for microscale sample preparation is described. Sample volumes of a few microliters can be handled in a contactless way and protected from the loss of analyte through adsorption, memory effects and contamination from container walls. Sample enrichment, derivatization and liquid/liquid-extraction can be performed in a single levitated droplet as part of the sample pretreatment in an analytical procedure. The levitation device follows a modular design and consists of an acoustic levitator, droplet size measurement, solvent and reagent supply and experiment control and documentation. The applicability of the experimental setup is demonstrated by the enrichment of hexachlorbenzene dissolved in isooctane and the derivatization of butyltin compounds with sodium tetraethylborate in water under subsequent extraction of the prealkylated products into hexane in droplets of 3 to 5 #mu#L volume as sample preparation for a gas-chromatographic separation. (orig.)SIGLEAvailable from TIB Hannover: RA 3251(99/E/75) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekDEGerman