Mechanismus orientierte Suche nach neuen Antitumorwirkstoffen aus Pflanzen. Teilprojekt 2: In-vitro und in-vivo Characterisierung an target-characterisierten Tumormodellen Abschlussbericht

Abstract

In a cooperative program with Prof. Dr. G. Eisenbrand, University of Kaiserslautern, the Oncotest GmbH evaluated the anticancer effects of novel compounds from plants in vitro against human tumor models and in vivo in nude mice. 57 new natural products out of the class of alcaloids (6), indoles (18), bisindoles (28) and flavones (5) were tested against 16-24 human tumors using the clonogenic assay. Based on the in vitro efficacy, 19 compounds were selected for in vivo studies against the large cell lung cancer LXFL 529 and partly against stomach and pancreas carcinomas. Six of the new compounds were highly effective in comparison to the control and effected a marked tumor inhibition. The comparison of the in vitro profile of the standard agents against the one of the standard compounds and the expression of potential molecular targets were done and a data base system was built. Models for metastases were developed for human tumors and characterized for the expression of the suppressor gene of metastases nm23 and the splice variant vCD44. These investigations were performed with carcinomas of the bladder, colon and pancreas which developed partly lung, liver and lymph node metastases. (orig.)SIGLEAvailable from TIB Hannover: DtF QN1(77,37) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung und Forschung (BMBF), Bonn (Germany)DEGerman