Mise au point d'une PCR quantitative de l'herpès virus humainde type 6 au laboratoire de virologie (intérêt dans le suivi des greffes de moelle osseuse)

Abstract

Human herpesvirus 6 primary infection usually happens in the first two years of life. Most of the time, it is harmless with only high fever and more rarely cutaneous rash. HHV-6 remains latent in numerous cell types and may reactivate in immunocompromised patients, especially those who undergo bone marrow or peripheral stem cell transplantation. HHV-6 infection is often associated with delayed engraftment or bone marrow suppression. It may also play a role in the outbreak or exacerbation of graft versus host disease. During this work, we developped a quantitative HHV-6 real-time PCR on the LightCycler instrument. It may efficiently help in determining HHV-6 reactivation and appreciating the effectiveness of antiviral therapy. In case of HHV-6 related symptoms, viral load quantification in blood and biopsy samples appear to be helpful to assess reactivation.TOULOUSE3-BU Santé-Centrale (315552105) / SudocSudocFranceF