Tn <i>FLXopen</i> : Markerless Transposons for Functional Fluorescent Fusion Proteins and Protein Interaction Prediction

Abstract

Transposon mutagenesis is a powerful tool for random mutagenesis, as insertion of a transposon and accompanying antibiotic resistance cassette often disrupt gene function. Here, we present a series of transposons with fluorescent protein genes which, when integrated in frame, may be selected with a fluorescence activated cell sorter (FACS).</jats:p