Modulation of carcinogen metabolism by benzyl isothiocyanate

Abstract

Precision-cut rat liver slices were incubated with various concentrations of benzyl isothiocyanate (BITC) (0-25 μM) for 24 hours. BITC decreased the dealkylation of ethoxy-(EROD) and methoxyresomfin (MROD) but increased CYP1A1/2 apoprotein levels; in vitro studies confirmed that it is mechanism-based inhibitor. BITC decreased both dealkylation of pentoxyresorufin (PROD) and CYP2B1 apoprotein levels but there was no effect on 7-benzyloxyquinoline (7-BQ) metabolism and CYP3A apoprotein levels. In the case of Phase II enzyme systems, BITC increased epoxide hydrolase and GST activity and a similar rises was observed in protein levels, no change was observed in NADPH:quinone oxidoreductase (NQO1).EThOS - Electronic Theses Online ServiceGBUnited Kingdo