Applications of RAPD Markers in Soybean: Genetic Diversity and Linkage Analysis

Abstract

143 p.Thesis (Ph.D.)--University of Illinois at Urbana-Champaign, 1999.To determine the efficacy of the pollen tube pathway transformation procedure plasmids carrying the bar and gus genes were applied to cut stigmas. Approximately 5000 seeds were produced from treated flowers. No plants derived from seeds produced by flowers treated with the plasmid carrying the bar gene were found to be as herbicide resistant as the positive control. Approximately 2% of seeds treated with the gus gene had a positive GUS reaction but primers specific for the gus gene failed to detect the gene.U of I OnlyRestricted to the U of I community indefinitely during batch ingest of legacy ETD