209 σ.Εθνικό Μετσόβιο Πολυτεχνείο--Μεταπτυχιακή Εργασία. Διεπιστημονικό-Διατμηματικό Πρόγραμμα Μεταπτυχιακών Σπουδών (Δ.Π.Μ.Σ.) “Δομοστατικός Σχεδιασμός και Ανάλυση των Κατασκευών”Η παρούσα εργασία έχει ως αντικείμενο τη μελέτη προσθήκης καθ' ύψος ενός ορόφου με μεταλλικό σκελετό σε υπάρχον διώροφο κτίριο από οπλισμένο σκυρόδεμα. Στα πλαίσια αυτής της μελέτης, απαιτήθηκε η προσομοίωση ολόκληρου του κτιρίου.The objective of the present work is the addition of a one story steel building to an existing two-story concrete building. In the scope of this project, the simulation of the complete building was required.Άννα Ν. Δημοπούλο

Ballingall

Belkaid

Bembridge

Bensaid

Binnington

Born

Brake

Brossard

Burger

Carvalho

Collins

Constantinoiu

Davis

George

Guerrero

Gutierrez

Jones

Jonsson

Kashino

Kemp

Keresztes

Kongsuwan

Koudstaal

Lahmers

Lew-Tabor

Liebana

Maxwell

Moorhouse

Morrison

Naessens

Niku

Piper

Riek

Roberts

Schleger

Stewart

Tatchell

Taylor

Utech

Verissimo

Wagland

Wikel

Willadsen

This study investigated the local immune response at larval attachment sites in Santa Gertrudis cattle with low and high levels of tick resistance. Skin samples with tick larvae attached were collected from Santa Gertrudis cattle at the end of a period of 25 weekly infestations, when the animals manifested highly divergent tick-resistant phenotypes. There was a tendency for more CD3+, CD4+, CD8+, CD25+, γδ T-cells and neutrophils to concentrate at larval tick attachment site in susceptible cattle than in resistant cattle but the differences were significant only for γδ T-cells and CD4+ cells. Most of the cattle developed intra-epidermal vesicles at the larval attachment site but the predominant cell within or around the vesicles was the neutrophil in susceptible animals and eosinophil in the resistant animals. The monoclonal antibodies (mAbs) specific for CD45 and CD45 RO antigens reacted with skin leukocytes from a higher number of susceptible cattle than resistant cattle. Our data suggest that some of the cellular responses mounted at larval attachment site are not involved in tick protection. The mAbs specific for CD45 and CD45 RO directly, or a test for CD45 genotype might be developed as markers of tick susceptibility or resistance

Constantinoiu, Constantin

Lew-Tabor, Ala

Jackson, Louise

Jorgensen, Wayne

Piper, Emily

Mayer, David G.

Johnson, Linda

Venus, Bronwyn

Jonsson, Nicholas

Queensland DAF eResearch Archive

Local immune response to larvae of Rhipicephalus microplus in Santa Gertrudis cattle

This study investigated the local immune response at larval attachment sites in Santa Gertrudis cattle with low and high levels of tick resistance. Skin samples with tick larvae attached were collected from Santa Gertrudis cattle at the end of a period of 25 weekly infestations, when the animals manifested highly divergent tick‐resistant phenotypes. There was a tendency for more CD3+, CD4+, CD8+, CD25+, γδ T cells and neutrophils to concentrate at larval tick attachment site in susceptible cattle than in resistant cattle but the differences were significant only for γδ T cells and CD4+ cells. Most of the cattle developed intra‐epidermal vesicles at the larval attachment site but the predominant cell within or around the vesicles was the neutrophil in susceptible animals and eosinophil in the resistant animals. The monoclonal antibodies (mAbs) specific for CD45 and CD45 RO antigens reacted with skin leucocytes from a higher number of susceptible cattle than resistant cattle. Our data suggest that some of the cellular responses mounted at larval attachment site are not involved in tick protection. The mAbs specific for CD45 and CD45 RO directly, or a test for CD45 genotype might be developed as markers of tick susceptibility or resistance

Constantinoiu, C.C.

Lew-Tabor, A.

Jackson, L.A.

Jorgensen, W.K.

Piper, E.K.

Mayer, D.G.

Johnson, L.

Venus, B.

Jonsson, N.N.

Research Repository

Aims:



This study investigated the local immune response at larval attachment sites in Santa Gertrudis cattle with low and high levels of tick resistance.

Methods and results:



Skin samples with tick larvae attached were collected from Santa Gertrudis cattle at the end of a period of 25 weekly infestations, when the animals manifested highly divergent tick-resistant phenotypes. There was a tendency for more CD3+, CD4+, CD8+, CD25+, γδ T-cells and neutrophils to concentrate at larval tick attachment site in susceptible cattle than in resistant cattle but the differences were significant only for γδ T-cells and CD4+ cells. Most of the cattle developed intra-epidermal vesicles at the larval attachment site but the predominant cell within or around the vesicles was the neutrophil in susceptible animals and eosinophil in the resistant animals. The monoclonal antibodies (mAbs) specific for CD45 and CD45 RO antigens reacted with skin leukocytes from a higher number of susceptible cattle than resistant cattle.

Conclusion:



Our data suggest that some of the cellular responses mounted at larval attachment site are not involved in tick protection. The mAbs specific for CD45 and CD45 RO directly, or a test for CD45 genotype might be developed as markers of tick susceptibility or resistance

Mayer, David

Enlighten

English

Enlighten: Publications

This study investigated the local immune response at larval attachment sites in Santa Gertrudis cattle with low and high levels of tick resistance. Skin samples with tick larvae attached were collected from Santa Gertrudis cattle at the end of a period of 25 weekly infestations, when the animals manifested highly divergent tick-resistant phenotypes. There was a tendency for more CD3+ , CD4+ , CD8+ , CD25+ , γδ T cells and neutrophils to concentrate at larval tick attachment site in susceptible cattle than in resistant cattle but the differences were significant only for γδ T cells and CD4+ cells. Most of the cattle developed intra-epidermal vesicles at the larval attachment site but the predominant cell within or around the vesicles was the neutrophil in susceptible animals and eosinophil in the resistant animals. The monoclonal antibodies (mAbs) specific for CD45 and CD45 RO antigens reacted with skin leucocytes from a higher number of susceptible cattle than resistant cattle. Our data suggest that some of the cellular responses mounted at larval attachment site are not involved in tick protection. The mAbs specific for CD45 and CD45 RO directly, or a test for CD45 genotype might be developed as markers of tick susceptibility or resistance

ResearchOnline at James Cook University

C. C. Constantinoiu

A. Lew-Tabor

L. A. Jackson

W. K. Jorgensen

E. K. Piper

D. G. Mayer

L. Johnson

B. Venus

N. N. Jonsson

Crossref

Parasite Immunology

Local immune response to larvae of<i>Rhipicephalus microplus</i>in Santa Gertrudis cattle

This study investigated the local immune response at larval attachment sites in Santa Gertrudis cattle with low and high levels of tick resistance.Skin samples with tick larvae attached were collected from Santa Gertrudis cattle at the end of a period of 25 weekly infestations, when the animals manifested highly divergent tick-resistant phenotypes. There was a tendency for more CD3+ , CD4+ , CD8+ , CD25+ , γδ T-cells and neutrophils to concentrate at larval tick attachment site in susceptible cattle than in resistant cattle but the differences were significant only for γδ T-cells and CD4+ cells. Most of the cattle developed intra-epidermal vesicles at the larval attachment site but the predominant cell within or around the vesicles was the neutrophil in susceptible animals and eosinophil in the resistant animals. The monoclonal antibodies (mAbs) specific for CD45 and CD45 RO antigens reacted with skin leukocytes from a higher number of susceptible cattle than resistant cattle.Our data suggest that some of the cellular responses mounted at larval attachment site are not involved in tick protection. The mAbs specific for CD45 and CD45 RO directly, or a test for CD45 genotype might be developed as markers of tick susceptibility or resistance. This article is protected by copyright. All rights reserved

University of Queensland eSpace

Local immune response to larvae of\ua0Rhipicephalus microplus\ua0in Santa Gertrudis cattle immune response against Rhipicephalus microplus

Design for the addition of a single steel framed story to a concrete building

Abstract

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