Deux nouveaux Mermithides, #Amphimermis ronderosi$ n. sp. et #A. dichroplusi$ n. sp., sont décrits et illustrés ; l'un et l'autre sont parasites de sauterelles récoltées dans la Province de Buenos Aires, Argentine. La première espèce se caractérise par : vagin long et en forme de S ; spicules enroulés de manière lâche sur les premiers 41% de la longueur, non enroulés sur les 14% suivants, de nouveau enroulés sur les 31% suivants et non enroulés sur les 14% postérieurs ; trois rangées de papilles génitales, la rangée ventrale comprenant 30 papilles préanales et 15 papilles postanales. #A. dichroplusi$ n. sp. est caractérisée par le vagin en forme de S ; les spicules non enroulés sur les premiers 6% de leur longueur, enroulés sur les 24% suivants, non enroulés sur les 12% suivants, de nouveau enroulés sur les 44% suivants et non enroulés sur les 14% postérieurs ; trois rangées de papilles génitales, la rangée ventrale comprenant 25 papilles préanales et 12 papilles postanales. (Résumé d'auteur

Camino, N.B.

Lange, C.E.

Horizon / Pleins textes

Fundam. appl. NemalOl., 1997,20 (3), 239-242
Two new species of the genus Amphimennis
Kaburaki & Imamura, 1932 (Nematoda: Mermithidae)
from Argentine grasshoppers (Orthoptera : Acrididae)
Nora B. CAMING and Carlos E. Lc\NGE
Centra de Estudios Pamsitot6gicos y de Veclorfs (CEPA VE),
Universidad Nacional de La PtataICON/CET, CaUe 2 fv7' 584, 1900 La Plata, Argentina.
Accepted for publication 8 May 1996.
Summary - Two new mermithids, Amphimermis ronderosi n. sp. and A. dichroplusi n. sp., are described and illustrated; both are
parasites of grasshoppers in Buenos Aires Province, Argentina. Diagnostic characters of the first species include: vagina long and
S-shaped; spicules loosely twisted for proximal 41 % of their length, untwisted for 14 %, again twisted for 31 %, and untwisted for
the last 14 %; three rows of genital papillae; the ventral row with 30 preanal and fifteen postanal papillae. The second species is
characterized by having: S-shaped vagina; the spicules untwisted for 6 % of their length, then twisted for 24 %, unt\-visted for 12 %,
twisted again for 44 %, and untwisted for the last 14 % of their length; three rows of genital papillae; the ventral row with 25 preanal
and twelve postanal papillae.
Resume - Deux nouvelles especes du genre Amphimermis Kaburaki & lrnarnura, 1932 (Nematoda: Merrnithidae)
parasites de sauterelles d'Argentine (Orthoptera : Acrididae) - Deux nouveaux Mermithides, Amphimermis ronderosi n. sp.
etA. dichroplusi n. sp., sont decrits et illustres; I'un et ['autre sont parasites de sauterelles recoltees dans la Province de Buenos Aires,
Argentine. La premiere espece se caracterise par: vagin long et en forme de S; spicules enroules de maniere lache sur les premiers
41 % de leur longueur, non enroules sur les 14 % suivants, de nouveau enrouJes sur les 31 % suivants et non enroules sur les 14 %
posterieurs; trois rangees de papilles genitales, la rangee ventrale comprenam 30 papilles preanales et IS papilles postanales. A.
dichro-plusi n. sp. est caracterisee par le vagin en forme de S; les spicules non enroules sur les premiers 6 % de leur ]ongueur, enroules
sur les 24 % suivants, non enroules sur les 12 % suivants, de nouveau enroules sur les 44 % suivants et non enrouJes sur les 14 %
posterieurs; trois rangees de papilles genitales, la rangee ventrale comprenant 25 papilles preanales et 12 papiJles postanales.
Key-words: Amphimerrnis, Argentina, grasshoppers, nematodes, new species.
While conducting field surveys on agricultural pests
in Argentina in areas of Brandsen and Olavarria (North-
east and center of Buenos Aires Province, respectively)
we found nymphs of grasshoppers that were parasitized
by two different Arnphirnennis species. Only one species
of Arnphirnermis, A. bonaerensis Miralles & Camino,
1983 was previously known parasitizing Argentine
grasshoppers, and its morphological characters marked-
ly differ from the two species here described as new.
Parasitized grasshoppers were placed in aluminium
cages with wire-screened walls. A shallow container with
sterilized moistened sand was provided, where post-par-
asitic nematodes migrated to mature. Adults and post-
parasitic juveniles were observed first alive and then
killed in distilled water at 60 QC for 2 min, fixed in T AF
and processed to glycerin by Seinhorst's method (Cur-
ran & Hominick, 1980). Living and fixed specimens
were employed for drawings and measurements using a
camera lucida microscope, and a micrometer in a Zeiss
compound microscope.
/SSN 1164-5571/97/03 S 7.00/ i£: GaUlhier-Villars - ORSTOM
Arnphirnennis ronderosi* n. sp.
(Fig. 1)
MEASUREMENTS
Female (paratypes; n = 12) : L = 67.5-120 (99.38 ±
19.71) mm; head diam. at cephalic papillae level = 56-
152 (123 ± 38.92) IJ..m; body diam. at nerve ring level =
120-220 (187.5 ± 40.85) IJ..m; max. body diam. = 234-
282 (2635 ± 19.46) IJ..m; body diam. at post. end of
trophosome level =200-255 (226.25 ± 26.31); dist. ant.
end to nerve ring = 300-350 (329 ± 21.75) IJ..m; V =
48-59 (52.47 ± 4.11); vagina length = 100-350
(239.5 ± 95.29) IJ..m; vagina width = 120-150
(134± 13.19) IJ..m.
Male (pararypes; n = 10): L = 18-72 (44.75 ±
23.97) mm; head diam. at cephalic papillae level = 50-
* In honor of Dr. R. A. Ronderos, an Argentinian specialist in
grasshoppers.
239
N. B, Camlno & C. E. Lange
Fig. 1. Amphimermis ronderosi n. sp. A: Head of the male,
ventral view; B : Head of the female, venlral view; C : Head of the
female, face vIew; D : Tail of the male, velllml view; E: Vagina;
F: Tail of the male, laleral view; G : Posl-parasilic juvenile wil;
H: Cross seclion al midbody. (Scale bars = 50 Mm).
-72 (61 ± 7.81) fLm; body diam. at nerve ring level =
100-180 (137.5 ± 30.31) fLm; max. body diam. = 160-
240 (197.5 ± 28.61) fLm; body diam. at anus level =
140-200 (182.5 ± 24.87) fLm; dist. ant. end to nerve
ring =190-210 (200 ± 7.07) fLm; dist. anus to tail =90-
288 (229 ±81.74) fLm; spicule length = 800-2400
(1562.5 ± 658.95) fLm; spicule width = 8-12 (10.25 ±
1.48) fLm.
Holotype (male) : L = 65 mm; head diam. at cephalic
papillae level =60 fLm; body diam. at nerve ring level =
150 fLm; max. body diam. = 200 fLm; body diam. at
anus level = 190 fLm; dist. ant. end to nerve ring =
210 fLm; dist. anus to tail = 250 fLm; spicule length =
2000 fLm; spicule width = 12 fLm.
Allotype (female) : L = 110 mm; head diam. at ce-
phalic papillae level = 144 fLm; body diam. at nerve ring
level = 220 fLm; max. body diam. = 280 fLm; body
diam. at post. end of tropbosome level = 250 fLm; dist.
ant. end to nerve ring = 316 fLm; V = 50; vagina
length =208 fLm; vagina width = 144 pm.
DESCRlPTION
Adults: Long white nematodes, male approximately
50 % of female length. Cuticle with crisscross fibres.
Head homocephalic. Mouth terminal and central. Six
cephalic papillae arround the mouth, at same level. Am-
phids small, cup-shaped. Six hypodermal chords, lateral
chords with three rows of cells along the body, dorsal
!\\!1\o
A B c ...
: :',
and ventral chords with one row of cells, and subventral
chord with two rows of cells.
Females: Vulva a longitudinal slit. Vulval flap absent.
Vulval cone present, base muscular. Vagina S-shaped,
long, muscular, anterior loop 33 % shorter than post-
erior loop.
Males: Spicule long, paired, loosely twisted for the
proximal 41 % of their length, then untwisted for 14 %,
twisted for 31 %, and fmally untwisted for 14 % of their
length. Tips fused, anenuated to fine point. Three rows
of genital papillae; the external rows with 30 papillae
each, arranged in single line; venu'al row with 30 preanal
and 15 postanal papillae in a single line.
TYPE HOST' AND LOCALITY
J\!Ietaleplea brevicornis (L.) (Orthoptera: Acrididae :
Acridinae), Brandsen, Province of Buenos Aires, Argen-
tina.
TYPE MATERlAL
Holotype and allotype deposited in the Helrnintho-
logical collection at CEPAVE and paratypes deposited
in the Invertebrate Division at Museo de La Plata, Ar-
gentina.
DIAGNOSIS AND RELATIONSHIPS
A. ronderosi n. sp. is characterized by the S-shaped
type of vagina, the twisting length of the spicule and the
arrangement of genital papillae in three single rows, the
ventral row having 30 preanal and 15 postanal papillae.
Baker and Poinar (1994) divided the genus into four
groups of species on the basis of the twisting of the
spicules and the shape of the amphids. The new species
belongs to the bogongae group, distinguished by having
spicules loosely t\visted at the distal and proximal halves
and untwisted in the middle, and cup-shaped amphids.
At present, the bogongae group is represented by the
species A. bogongae Welch, 1963, a parasite of Lepi-
doptera Noctuidae from Australia; A. uny Nickle, 1972,
found in damsel flies from the USA; A. maritima Rub-
zov, 1971, from an unknown host in Russia; A. lilOrahs
Artyukbovski & Karchenko, 1971, also from an un-
known Russian host; A. bonaerensis Miralles & Camino,
1983, a parasite of acridids from Argentina; and A. mi-
rabinda Baker & Poinar, 1994 found in acridids from
Australia.
The male of A. ronderosi n. sp. differs from all known
species by the twisted length of the spicules and the
number and arrangement of the genital papillae. Spicule
length of A. ronderosi n. sp. is comparable to that of
A. mirabinda, A. bonaerensis and A. maritima. However,
A. mirabinda can be distinguished from the new species
in having enormous amphids (width approximately half
head diameter). A. bonaerensis is separated from the new
species in the twisting and untwisting percentage of the
spicule length (31, 14, and 41 % vs 28, 12,28 %) and
240 Fundam. appl. Nematol.
Two new Amphimermis
DIAGNOSIS AND RELATIONSHIPS
A. dichmplusi n. sp. is characterized by the configu-
ration of the S-shaped vagina, the rwisting length of the
Fig. 2. Amphimermis dichroplusi n. sp. A : Head of male, ven-
lral view; B : Head of female, ventral view; C: Head offemale,
face view; D : Tail of male, ventral view; E: Vagina; F: Tail of
male, laleral View; C : Cross seclion al midbody; H : Posl-parasil-
ic Juvenile wil; I: Tip of lhe spiatles. (Scale ban = 50 j.Lm).
E
c
B
A
ic. Mouth terminal and central. Six cephalic papillae
arranged in a circle arround the mouth. Amphids medi-
um-sized, cup-shaped. Six hypodermal chords; lateral
chords containing four rows of cells at midbody and
three rows of celJs at the anterior and posterior ends of
the body; dorsal chord with one row of celJs; ventral and
subventral chords each with t'NO rows of cells.
Females: Opening of vulva a transverse slit. Vulval
flap present ressembling rwo large lips. Vulval cone ab-
sent. Vagina S-shaped, long, muscular, anterior loop
approximately 10 % of the length of posterior loop.
Males: Spicules long, paired. Anterior end untwisted
for 6 %, then loosely t\Nisted for 24 %, unt\visted for
12 %, rwisted for distal44 %, and finally unrwisted in the
posterior 14 %. Tips fused, wedge-shaped. Three rows
of genital papillae; the external rows each with 21 papil-
lae arranged in a single Line; ventral row with 25 preanal
and 12 postanal papillae in a single line.
TYPE HOST fu"1D LOCALITY
Dichroplus elongatus Giglio-Tos, 1894 (Orthoptera:
Acrididae: Melanoplinae). Olavarria, Province of Bue-
nos Aires, Argentina.
TYPE ,v1.ATERIAL
Holorype and allorype deposited in the helmintholo-
gical collection at CEPAVE and pararypes deposited in
the Invertebrate Division at Museo de La Pia ta, Argenti-
na.
Arnphimennis dichroplusi* n. sp.
(Fig. 2)
MEASUREMENTS
Female (pararypes; n = 15) : L = 230-370 (292.5 ±
51.17) mm; head diam. at cephalic papillae level = 61.1-
66 (63.72 ± 1.68) f-lm; body diam. at nerve ring level =
129-156 (136.42 ± 11.31) f-lm; max. body diam. = 348-
364 (355 ± 5.74) f-lm; body diam. at post. end of tro-
phosome level =244-300 (263.5 ± 21.83) f-lm; dist. ant.
end to nerve ring = 300-340 (312 ± 16.50) f-lm; V =
49-54 (51 ± 1.87); vagina length = 740-2980 (1668 ±
895.18) f-lm; vagIna width = 104-268 (186.5 ±
76.7) f-lm; length and width ofamphids =21-24 (22.75
± 1.3) x 16-20 (18 ± 1.41) f-lm.
Male (pararypes; n = 12) : L = 46-65
(51.75 ± 7.82) mm; head diam. at cephalic papillae lev-
el = 55-68 (60.35 ± 5.13) f-lm; body diam. at nerve ring
level =82-120 (94.44 ± 15.02) f-lm; max. body diam. =
150-200 (173.18 ± 22.12) f-lm; body diam. at anus lev-
el = 110.45-190 (147.61 ± 28.47) f-lm; dist. ant. end to
nerve ring = 210-260 (240 ± 18.71) f-lm; dist. anus to
tail = 190-220 (205 ± 11.18) f-lm; spicule length =
1600-2000 (1806 ± 141.80) f-lm; spicule width = 12-14
(13.05 ± 1.05) f-lm; length and width of amphids = 20-
22 (20.84 ± 0.84) x 7-9 (8 ± 0.68) f-lm.
Holotype (male) : L =50 mm; head diam. at cephalic
papillae level =68 f-lm; body diam. at nerve ring level =
90 f-lm; max. body diam. = 150 j-lm; body diam. at anus
level = 150 j-lm; dist. ant. end to nerve ring = 260 f-lm;
dist. anus to tail =210 f-lm; spicule length = 1800 f-lm;
spicule width = 12 j-lm.
Allotype (female) : L = 270 mm; head diam. at ce-
phalic papillae level =64 f-lm; body diam. at nerve ring
level = 130.5 f-lm; max. body diam. = 354 f-lm; body
diam. at post. end of trophosome level = 260 f-lm; dist.
ant. end to nerve ring = 308 f-lm; V = 50; vagina
length = 2000 f-lm; vagina width = 258 f-lm.
DESCRIPTION
Adults: Long, white nematodes, male 17 % of female
length. Cuticle with crisscross fibres. Head homocephal-
" From the generic name of the hose, Dichroplus elongams.
A. maritima differs in male body length (18-72 vs 25-
102 mm).
Female A. rondemsi n. sp. is distinguished from the
other species of the group by the rype of S-vagina (with
anterior loop 33 % shorter than the posterior loop) and
differs further from A. bogongae and A. bonaerensis in the
length of the vagina (100-350 vs 160-205 and 51-
113 f-lm, respectively); the new species has shorter vagi-
na length compared to that of A. mirabinda (100-350 vs.
380-850 f-lm long).
Vol. 20, n° 3 - 1997 241
N. B. Camino & C. E. Lange
spicule and the arrangement of genital papillae in three
rows, the ventral row having 25 preanal and 12 postanal
papillae.
A. dichroplusi n. sp. belongs to the bogongae group
(Baker & Poinar, 1994) because of the spicule loosely
twisted at the distal and proximal halves and untwisted
in the middle, and the cup-shaped amphids. The male
of A. dichmplusi n. sp. is distinguished from ail previous-
ly described species of the bogongae group (except
A. mirabinda) by the length of the spicules, the new
species having shorter spicuJes (1600-2000 fLm). A. mi-
rabinda differs from A. dichmplusi n. sp. in having enor-
mous amphids, which are larger in both sexes. A. di-
chroplusi n. sp. can be distinguished from A. bonaerensis
in the number of ventral genital papillae (25 preanal and
twelve postanal vs 28-16). The new species also differs
from A. bogongae in the percentage of the twisted and
untwisted spicule length (24, 12,44, and 14 % vs 24, 24,
16, and 36 %, respectively). The female of A. dichroplusi
n. sp. can be separated from all the other species of the
group bogongae by having a long (740-2980 fLm) and
S-shaped vagina, with a ftrst loop 90 % shorter than the
second loop.
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Two new species of the genus Amphimermis Kaburaki and Imamura, 1932 (Nematoda : Mermithidae) from Argentine grasshoppers (Orthoptera : Acrididae)

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