Ahmad, I.

Ahmad, W.

Horizon / Pleins textes

Fundam. app/. NemalO/., 1992,15 (2), 149-152.
Makatinus heynsi n. Sp. (Dorylaimida : Aporcelaimidae)
from Goa, India.
Wasim AHMAD and Irfan AHMAD
Section of Nemat%gy, Department of Zo%gy, A/igarh Mus/im University, A/igarh-202002, India.
Accepted for publication 6 February 1991.
Summary - Makalinus heynsi n. sp. is described and illustrated. It is characterized by : L = 1.96-2.42 mm; a ~ 37-43;.b =
4-5; c = 82-110; V = 46-52; odontostyle = 21-25 J.lm; odontophore = 33-38 J.lm; spicules = 57-67 J.lm. M. heyns! n. sp. dlffers
from the other species in having a shorter body and a transverse vulva.
Résumé - Makatinus heynsi n. sp. (Dorylaimida : Aporcelaimidae) provenant de Goa, Inde - Makalinus heynsi n. sp.
est décrit et figuré. Il est caractérisé par: L. = 1,96-2,42 mm; a = 37-43; b = 4-5; c = 82-110; V = 46-52; odontostyle =
21-25 um' odontophore = 33-38 J.lm; spicules = 57-67 J.lm. M. heynsi n. sp. diffère des autres espèces du genre par un corps plus. ,
court et la vulve en fente transversale.
Key-words : Makalinus.
A soil sample collected from around the roots of
paddy near Mangeshi temple, Goa, yielded a large
number of specimens of an unknown dorylaim nema-
tode. Because of the presence of a truck cuticle with fine
punctations, a large odontostyle with aperture about
50 % of its length, unsclerotized (plicated) guiding ring
and two pairs of adanal supplements, it fits best in the
genus Makatinus Heyns, 1965 (Aporcelaimidae : Dory-
laimoidea). The present species has a transverse vulva
instead of a longitudinal one reported in the three known
species. However, the presence of a transverse or longi-
tudinal vulva in members of the same genus is common
in Dorylaimoidea (MesodorylaimusJ Eudorylaimus, etc.).
The shape of lip region and odontostyle and the ar-
rangement of supplements is similar to Makatinus es-
pecially M. puncealUs Heyns, 1965, the type species.
Hence the present species is placed in the genus Maka-
tinus and described as M. heynsi n. sp.
The genus Makatinus differs from Aporcelaimus
Thome & Swanger, 1936 mainly in having an amalga-
mated lip region (except in M. macropuncealUs), punc-
tate cuticle, longitudinal vulva, absence of cardiac disc
and the presence of two adanal pairs of supplements
(except in M. macropuncealUs). The present species
though having a transverse vulva cannot be accomo-
dated in Aporcelaimus because of the shape of lip region,
punctations on cuticle, absence of cardiac disc and the
nature of adanal supplements (lip region distinctly set
off, cuticle with criss-cross lines, cardiac disc present
and a single pair of adanal supplements in Aporcelai-
mus).
Ali three known species of MakatinusJ viz., M. punc-
talUs Heyns, 1965, M. capensis Heyns, 1965, and M.
macropuncealUs Heyns, 1967 have been reported from
South Africa. M. heynsi n. sp. represents the first species
being reported from outside Africa.
Materia1s and methods
Specimens for light microscopy were killed and flXed
in hot 4 % formai in, dehydrated by the slow method and
mounted in glycerin. Measurements were made with an
ocular micrometer. For SEM, formalin flXed specimens
were washed in buffer, post-flXed in osmium tetroxide,
dehydrated in an alcohol series and critical point dried in
COz' Dried specimens were mounted on aluminium
stubs and coated with 30 nm gold and observed on a
Hitachi S 2300 SEM at 15 kY.
Makatinus heynsi * n. sp.
(Fig. 1 & 2)
DIMENSIONS
Female (Paratypes; n 10). L 1.96-2.42
(2.25) mm; a = 37-43 (39); b = 4.0-5.0 (4.5); c =
82-110 (92); c' = 0.62-0.72 (0.67); V = 46-52 (50);
G, = 11-14 (13); G z = 9-14 (12); odontostyle = 21-25
(23) llm; odontophore = 33-38 (37) llm; oesophagus =
472-518 (499) llm; prerectum = 114-156 (130) llm;
rectum = 32-40 (36) llm; tail = 22-28 (25) llm; ABD =
35-41 (37) llm.
Male (Paratypes; n = 5). L = 2.04-2.29 (2.16) mm;
a = 39-43(41);b = 4.3-4.8 (4.5); c = 71-90 (78); c' =
0.62-0.80 (0.70); T = 45-57 (52); odontostyle = 20-23
(22) llm; odontophore = 35-38 (37) llm; oesophagus =
462-501 (476) llm; spicules = 57-67 (64) llm; lateral
guiding pieces = 14-15 (15) llm; ventromedian sup-
plements = 9-11 (11); prerectum = 157-172 (160) llm;
tail = 26-30 (28) llm; ABD = 35-39 (37) llm.
Holotype (female). L = 2.17 mm; a = 41; b = 4.1;
c = 85; c' = 0.62; V = 52; G 1 = 13; Gz = 12j
* Named after Prof. J. Heyns in recognition of his work on
aporcelaimid nematodes.
ISSN 1164-5571/92/02/14904 $ 2.40/ © Gauthier-Vil/ars - ORSTOM 149
86-87
88-89
W Ahmad and 1. Ahmad
odontostyle = 25 J.1m; odontophore = 36 J.1m; oesopha-
gus = 518 Ilm; prerectum = 126 Ilm; rectum =
35 Ilm; tail = 26 Ilm; ABD = 41 Ilm.
DESCRIPTION
Female : Body ventrally curved upon flXation, taper-
ing anteriorly from base of oesophagus. Cuticle finely
striated, its thickness 3-5 Ilm at midbody and 5-6 Ilm at
tail. Each stria 0.7 !lm apart, bearing fine punctations.
Punctations distinct tawards extremities, more so in the
posterior region. Radial striae absent. Lateral chords
about one fourth of body width at midbody. Lateral,
dorsal and ventral papillae distinct under SEM. Lip
region set off by a slight depression, 15-16 !lm wide and
5-6 !lm high. Oral aperture a dorso-ventral slit sur-
rounded by six closely amalgamated equal-sized lips.
Labial papillae arranged in a 6 + 4 + 6 pattern. Am-
phids stirrup shaped, their apertures 10-11 Ilm or about
two thirds of the corresponding body width. Odontostyle
1.4-1.7 lip region widths long, its aperture about half of
its length. Guiding ring at 10-12 Ilm or about 0.7-0.8 lip
region widths from anterior end. Odontophore simple,
rod-like, 1.4-1.8 times the odontostyle length. Nerve
ring at 152-183 !lm from anterior end of body. Oesopha-
geaI expansion graduaI, expanded part occupying
44-45 % of total oesophageal length. Cardia elongate
conoid, 15-18 !lm long. Oesophageal gland nuclei and
their orifices located as follows :
DO = 50-52 SINI 68-70
DN = 54-55 SINz = 75-76
DO-DN = 3-4
Reproductive system amphidelphic. Vulva a trans-
verse slit, 8-9 Ilm wide, vagina 28-30 Ilm or about half
of corresponding body width. Two triangular, strongly
cuticularized pieces present at the vulva-vagina junc-
tion. Both sexual branches equally developed. Prerec-
tum about 3-4 anal body widths long. Rectum about one
anal body width long. Anus a short transverse slit about
8 Ilm wide. Tail short, hemispheroid, 0.6-0.7 anal body
widths long with a pair of caudal papillae on each side.
Male : Similar ta females in general morphology
except for a more curved posterior region. Spicules
dorylaimoid, 1.6-1.8 anal body widths long. Lateral
guiding pieces about one fourth of spicule length.
Supplements poorly developed, arranged in a demar-
cated midventral area. Ventromedians 9-11 in number,
regularly spaced. Adanals consisting of two pairs about
7 Ilm apart. Six or seven ventrolateral papillae on both
sides of the supplement region. Prerectum about four
anal body widths long. Anus crescent-shaped, about
8 Ilm wide, with a slighùy swollen posterior lip. Tail
short conoid with a bluntly rounded terminus, about
0.6-0.7 anal body widths long with three caudal papillae
on each side.
TYPE MATERIAL
Holotype : Female (and a paratype male) on slide
150
Makatinus heynsi n. sp./I in the nematode collection of
the Department of Zoology, A1igarh Muslim University
A1igarh. '
Paratypes : Ten females and four males on slides M.
heynsi n. sp./2-8; in the same collection. A paratype
female and a male deposited at Muséum National
d'Histoire Naturelle, Laboratoire des Vers, Paris,
France.
TYPE HABITAT AND LOCALITY
Soil around roots of paddy, Oryza sativa L., from
Mangeshi, Goa, India. Collected in April, 1982.
DIAGNOSIS AND RELATIONSHIP
Makatinus heynsi n. sp. differs from ail the known
species of Makatinus Heyns, 1965 in having a smaller
body and a small transverse slit-like vulva.
It further differs from M. punetatus in having a
slightly shorter odontostyle, anteriorly located vulva,
differently shaped tail, smaller spicules and smaller
number of ventromedian supplements CL
2.4-3.1 mm; odontostyle = 25-27 Ilm; V = 56-59;
spicules = 75-80 J.1m; ventromedian supplements =
21-23; vulva longitudinal and tail convex-conoid with a
prominent hyaline tip in M. punetatus). From M.
macropunctatus Heyns, 1967 it differs in shape of lip
region, in having weaker cuticular punctations, a smaller
c value, smaller spicules and in the arrangement of
ventromedian supplements CL = 3.19 mm; cuticular
punctations prominent; c = 133; spicules = 76-80 Ilm;
lip region offset by a constriction, lips not closely
amalgamated and adanal supplements a single pair in M.
macropunetatus).
Key to species of Makatinus Heyns, 1965
1. - Lip region offset by a deep constriction; lips COnspic-
uously separated; punctations prominent (9 : L =
3.19mm;a = 38;b = 4.3;c = 133;V = 54.5; cf :L =
2.76-2.80 mm; a = 39-42; b = 3.8-4.1; c = 91-118;
odontosryle = 26 J.1m; spicules = 76-80 J.1m; supple-
ments = 10 + 2) macropllnctaws Heyns, 1967
- Lip region offset by a depression or slight constriction,
lips amalgamated; punctations weak 2
2. - Vulva transverse (9 : L = 1.9-2.4 mm; a = 37-43; b =
4.0-5.0; c = 82-110; V = 46-52; cf : L = 2.0-2.3 mm; a =
39-43; b = 4.3-4.8; c = 71-90; odontosryle = 21-25 um;
spicules = 57-67 J.1m; supplements = 9-11 + 2 .; 2)
.................................. heynsi n. sp.
- Vulva longitudinal 3
3. - Tail convex-conoid with digitate tiPi vulva post-equa-
torial (9 : L = 2.4-3.1 mm; a = 36-42; b = 4.2-4.4; c =
84-92; V = 56-59; cf : L = 2.8-3.6 mm; a = 40-45; b =
4.7-4.9; c = 89-91; odontosryle = 25-27 ~; spicules =
75-80 J.1m; supplements = 19 + 2 + 2) ..
................. pllnceaws Heyns, 1965
Tail short, bluntly rounded; vulva median (9 : L =
FlIndam. appl. NemalOl.
lYtakatinus heynsi n. sp.
20 IJm
1---------'''-------;1 A, C
50 IJmI-----::....::....!::.:..:..:...---ll B, 0 -F
Fig. 1. Makarinus heynsi n. sp. A : Anterior region; B : Expanded part of oesophagus; C : Oesophago-intestinal junction; D : Female
gonad (anterior branch); E : Female posterior region; F : Male posterior region.
3.6-3.7 mm; a = 32-34; b = 4.4-5.0; c = 103-107; V =
48-50; odontostyle = 25-27 ).lm; males not known) .
............................. capensis Heyns, 1965
Vol. 15, nO 2 - 1992
Acknowledgments
This work was carried out under the Departmental Research
Support Programme of the University Grants Commission.
151
W. Ahmad and 1. Ahmad
Fig. 2. Makalinus heynsi n. sp. A, B : Face viewsi C : Anterior end; D : Male posterior region showing supplements; E : Body
striations showing punctations; F : Vulva; G : Male cloacal region showing adanal supplements and first ventromedian supplements,
situated in a demarcated midventral area. (Bar is equal w 5 {Lm in A, B, C, D, F; 2 {Lm in E and 10 {Lm in G.)
References
HEYNS, J. (1965). On me morphology and taxonomy of me
Aporcelaimidae, a new family of dorylaimid nematodes.
Enlom%gy Memoirs, Depal1.menl of Agricullura/ Technica/
Seroices, Republic of Soulh Africa, 10 : 5 1 p.
152
HEYNS, J. (1967). Makalinus macropunClalUS n. sp. (Nema-
toda : Aporcelaimidae) from the Kruger National Park.
Koedoe, JO : 101-103.
Fundam. appl. Nemaw/.


Makatinus heynsi n. sp. (Dorylaimida : Aporcelaimidae) from Goa, India

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