Разработаны схемы активных СВЧ-фильтров, пригодных для исполнения в виде гибридной или полупроводниковой микросхемы

F Cumhur Oner

Jacqueline Alblas

Michiel Croes

Moyo C Kruyt

Okan Bastian

Taco J Blokhuis

Wouter J A Dhert

Технология и конструирование в электронной аппаратуре

PubMed

Филинюк, Н.А.

Куземко, А.М.

Наукова електронна бібліотека періодичних видань НАН України (Vernadsky National Library of Ukraine)

Схемотехническое моделирование и синтез активных СВЧ-фильтров на полевых транзисторах Шоттки

Several inflammatory processes underlie excessive bone formation, including chronic inflammation of the spine, acute infections, or periarticular ossifications after trauma. This suggests that local factors in these conditions have osteogenic properties. Mesenchymal stem cells (MSCs) and their differentiated progeny contribute to bone healing by synthesizing extracellular matrix and inducing mineralization. Due to the variation in experimental designs used in vitro, there is controversy about the osteogenic potential of proinflammatory factors on MSCs. Our goal was to determine the specific conditions allowing the pro-osteogenic effects of distinct inflammatory stimuli. Human bone marrow MSCs were exposed to tumor necrosis factor alpha (TNF-α) and lipopolysaccharide (LPS). Cells were cultured in growth medium or osteogenic differentiation medium. Alternatively, bone morphogenetic protein 2 (BMP-2) was used as osteogenic supplement to simulate the conditions in vivo. Alkaline phosphatase activity and calcium deposition were indicators of osteogenicity. To elucidate lineage commitment-dependent effects, MSCs were pre-differentiated prior treatment. Our results show that TNF-α and LPS do not affect the expression of osteogenic markers by MSCs in the absence of an osteogenic supplement. In osteogenic differentiation medium or together with BMP-2 however, these mediators highly stimulated their alkaline phosphatase activity and subsequent matrix mineralization. In pre-osteoblasts, matrix mineralization was significantly increased by these mediators, but irrespective of the culture conditions. Our study shows that inflammatory factors potently enhance the osteogenic capacity of MSCs. These properties may be harnessed in bone regenerative strategies. Importantly, the commitment of MSCs to the osteogenic lineage greatly enhances their responsiveness to inflammatory signals

Croes, Michiel

Oner, F Cumhur

Kruyt, Moyo C

Blokhuis, Taco J

Bastian, Okan

Dhert, Wouter J A

Alblas, Jacqueline

Regenerative Medicine, Stem Cells & Cancer

Faculteit Diergeneeskunde

dES RMSC

NARCIS 

Proinflammatory Mediators Enhance the Osteogenesis of Human Mesenchymal Stem Cells after Lineage Commitment

Utrecht University Repository

Directory of Open Access Journals

PLoS ONE

Proinflammatory Mediators Enhance the Osteogenesis of Human Mesenchymal Stem Cells after Lineage Commitment.

http://dspace.nbuv.gov.ua/help/2015_Statistic.pdf

Схемотехническое моделирование и синтез активных СВЧ-фильтров на полевых транзисторах Шоттки

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Наукова електронна бібліотека періодичних видань НАН України (Vernadsky National Library of Ukraine)

NARCIS

Utrecht University Repository

Directory of Open Access Journals