Інститут проблем моделювання в енергетиці ім. Г.Є. Пухова НАН України
Doi
Abstract
A real time quantitative PCR combined with propidium monoazide (PMA) treatment of samples was
implemented to quantify live C. jejuni, C. coli and C. lari on broiler chicken carcasses at selected processing
steps in the slaughterhouse. The samples were enumerated by culture for comparison. The Campylobacter
counts determined with the PMA-qPCR and the culture method were not concordant. We conclude that
the qPCR combined with PMA treatment of the samples did not fully reduce the signal from dead cells